Evidence map›Paper›PMID 31541516›Full record

ArticleOral diseases2019

Influence of T2DM and prediabetes on blood DC subsets and function in subjects with periodontitis.

Mariana de Sousa Rabelo, Ahmed El-Awady, Adriana Moura Foz, Giovane Hisse Gomes, Mythilpriya Rajendran, Mohamed M Meghil, Scott Lowry, Giuseppe Alexandre Romito, Christopher W Cutler, Cristiano Susin

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Oral diseases, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Mariana de Sousa RabeloDivision of Periodontics, Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo, Brazil.ORCID https://orcid.org/0000-0003-1385-8471
Ahmed El-AwadyDepartment of Periodontics, The Dental College of Georgia, Augusta University, Augusta, GA, USA.
Adriana Moura FozDivision of Periodontics, Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo, Brazil.
Giovane Hisse GomesDivision of Periodontics, Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo, Brazil.
Mythilpriya RajendranDepartment of Periodontics, The Dental College of Georgia, Augusta University, Augusta, GA, USA.
Mohamed M MeghilDepartment of Periodontics, The Dental College of Georgia, Augusta University, Augusta, GA, USA.ORCID https://orcid.org/0000-0003-2344-3579
Scott LowryDepartment of Periodontics, The Dental College of Georgia, Augusta University, Augusta, GA, USA.
Giuseppe Alexandre RomitoDivision of Periodontics, Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo, Brazil.ORCID https://orcid.org/0000-0001-5669-912X
Christopher W CutlerDepartment of Periodontics, The Dental College of Georgia, Augusta University, Augusta, GA, USA.
Cristiano SusinDepartment of Periodontology, Adams School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Augusta University · USUniversidade de São Paulo · BRUniversity of North Carolina at Chapel Hill · US

Funding

Oral Pathogens and Dendritic Cell SubsetsR01DE014328 · NIDCR · STATE UNIVERSITY NEW YORK STONY BROOK · PI CUTLER, CHRISTOPHER WILLIAM · 2001 to 2014
$5.1M
Peripheral blood dendritic cells and periodontitisR21DE020916 · NIDCR · STATE UNIVERSITY NEW YORK STONY BROOK · PI CUTLER, CHRISTOPHER WILLIAM · 2011 to 2012
$362k
Foundation for the National Institutes of Health R01 DE014328 & R21 DE020916Fundação de Amparo à Pesquisa do Estado de São Paulo 2011/06982-4NIDCR NIH HHS R01 DE014328NIDCR NIH HHS R21 DE020916
6 · The paper itself

Abstract

objectiveTo compare the myeloid and plasmacytoid DC counts and maturation status among subjects with/without generalized periodontitis (GP) and type 2 diabetes mellitus (T2DM).

methodsThe frequency and maturation status of myeloid and plasmacytoid blood DCs were analyzed by flow cytometry in four groups of 15 subjects: healthy controls, T2DM with generalized CP (T2DM + GP), prediabetes with GP (PD + GP), and normoglycemics with GP (NG + GP). RT-PCR was used to determine levels of Porphyromonas gingivalis in the oral biofilms and within panDCs. The role of exogenous glucose effects on differentiation and apoptosis of healthy human MoDCs was explored in vitro.

resultsRelative to controls and to NG + GP, T2DM + GP showed significantly lower CD1c + and CD303 + DC counts, while CD141 + DCs were lower in T2DM + GP relative to controls. Blood DC maturation required for mobilization and immune responsiveness was not observed. A statistically significant trend was observed for P. gingivalis levels in the biofilms of groups as follows: controls <NG+GP < PD+GP < T2DM+GP. Moreover, significantly higher P. gingivalis levels were observed in blood DCs of NG + GP than controls, whereas no differences were observed between controls and PD + GP/T2DM + GP. In vitro differentiation of MoDCs was significantly decreased, and apoptosis was increased by physiologically relevant glucose levels.

conclusionType 2 diabetes mellitus appears to inhibit important DC immune homeostatic functions, including expansion and bacterial scavenging, which might be mediated by hyperglycemia.

Indexed as

Diabetes Mellitus, Type 2PeriodontitisPrediabetic StateAdultAgedBrazilDendritic CellsHumansMiddle AgedPorphyromonas gingivalisblood dendritic cellsperiodontitisporphyromonas gingivalisprediabetestype 2 diabetes

Identifiers

PMID31541516
PMCPMC6933074
OpenAlexW2974327777

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.