ArticleScientific reports2019
PCR-based Sepsis@Quick test is superior in comparison with blood culture for identification of sepsis-causative pathogens.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Fast Microbiology: A Pilot Study Comparing Culture-Independent Digital PCR and Blood Culture for Pathogen Detection in Sepsis.International journal of molecular sciences · 2026Article
- Validation of CRownLab Pneumoplex for the Detection ofInternational journal of microbiology · 2026Article
- Point-of-Care Testing and Biomarkers in Biliary Diseases: Current Evidence and Future Directions.Journal of clinical medicine · 2025Review
- Isolation and identification of bacteria from blood within 12 h using standard laboratory equipment.Scientific reports · 2025Article
- Electrochemical Aptamer-Based Biosensors for Sepsis Diagnosis: Recent Advances, Challenges, and Future Perspectives (2020-2025).Biosensors · 2025Review
- From Tradition to Innovation: Diverse Molecular Techniques in the Fight Against Infectious Diseases.Diagnostics (Basel, Switzerland) · 2024Review
- Advances in Host Depletion and Pathogen Enrichment Methods for Rapid Sequencing-Based Diagnosis of Bloodstream Infection.The Journal of molecular diagnostics : JMD · 2024Review
- Identifying antibiotic-resistant strains via cell sorting and elastic-light-scatter phenotyping.Applied microbiology and biotechnology · 2024Article
- Detection ofMicrobiology spectrum · 2024Article
- Spectrum and antimicrobial resistance in acute exacerbation of chronic obstructive pulmonary disease with pneumonia: a cross-sectional prospective study from Vietnam.BMC infectious diseases · 2024Article
- Broviac Catheter-Related Aortic Valve Infective Endocarditis Complicated With Massive Aortic Regurgitation Requiring Emergency Surgery: A Case Report.Case reports in infectious diseases · 2024Article
- Nano-Biotechnology for Bacteria Identification and Potent Anti-bacterial Properties: A Review of Current State of the Art.Nanomaterials (Basel, Switzerland) · 2023Review
- Antimicrobial Stewardship on Patients with Neutropenia: A Narrative Review Commissioned by Microorganisms.Microorganisms · 2023Review
- Relationship between antibiotic consumption pattern and antibiotic resistance in neonatal sepsis.Medicine and pharmacy reports · 2023Article
- Predictive Score for Carbapenem-Resistant Gram-Negative Bacilli Sepsis: Single-Center Prospective Cohort Study.Antibiotics (Basel, Switzerland) · 2022Article
- Diagnosis of pathogens causing bacterial meningitis using Nanopore sequencing in a resource-limited setting.Annals of clinical microbiology and antimicrobials · 2022Article
- Identification of potential risk factors for the poor prognosis of neonatal sepsis.Medicine and pharmacy reports · 2022Article
- The characteristics of bacteremia among patients with acute febrile illness requiring hospitalization in Indonesia.PloS one · 2022Article
- Circulating miR-147b as a diagnostic marker for patients with bacterial sepsis and septic shock.PloS one · 2021Article
- miRNAs as Biomarkers in Disease: Latest Findings Regarding Their Role in Diagnosis and Prognosis.Cells · 2020Review
Corrections and comments
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4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sepsis is an acute, often fatal syndrome that requires early diagnosis and proper treatment. Blood culture (BC) is the gold standard for the identification of pathogens, however it has marked limitations, including that it is time-consuming (delaying treatment) and can only detect microbes that readily grow under culture conditions. Alternatively, non-culture-based methodologies like polymerase chain reaction (PCR) are faster but also have limitations; e.g., the reaction is often inhibited by the abundance of human DNA and thus can only detect limited known target pathogens. In our previous publication, we have demonstrated a proof-of-concept of a simple pre-analytical tool to remove human DNA from patients' blood specimens, hence allowing downstream PCRs to detect rare bacterial genetic materials. In the current study, we reported a better performance of a novel prototype diagnosis kit named Sepsis@Quick that combines human DNA removal step with real-time PCRs compared to blood-culture for identifying sepsis causative bacteria. Our data showed that Sepsis@Quick is superior to blood culture in which the novel diagnostic kit could identify more pathogens and even polymicrobial infection, faster and less influenced by the empirical administration of broad spectrum antibiotic therapy (single administration or combination of cephalosporin III and fluoroquinolon). Additionally, for the first time, we demonstrated that positive results achieved by Sepsis@Quick are significantly associated with a reduction of sepsis-related mortality.
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