ArticleScientific reports2019
RNA-based qPCR as a tool to quantify and to characterize dual-species biofilms.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Phytosphingosine disrupts dual-species oral biofilms and attenuates LPS-induced pro-inflammatory responses in human gingival fibroblasts.Scientific reports · 2026Article
- Setting higher standards for reports of microbial species in human cancers.Nature cancer · 2026Review
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- A novel ionic liquid-based approach for DNA and RNA extraction simplifies sample preparation for bacterial diagnostics.Analytical and bioanalytical chemistry · 2024Article
- Novel delivery systems for controlled release of bacterial therapeutics.Trends in biotechnology · 2024Review
- Ecology ofBiofilm · 2024Review
- A review of mechanism analysis methods in multi-species biofilm of foodborne pathogens.Food science and biotechnology · 2023Review
- Alterations in Antibiotic Susceptibility ofInternational journal of molecular sciences · 2023Article
- Gene Expression Quantification from Pathogenic Bacterial Biofilms by Quantitative PCR.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Imaging biofilms using fluorescenceFrontiers in cellular and infection microbiology · 2023Review
- Biomass-specific rates as key performance indicators: A nitrogen balancing method for biofilm-based electrochemical conversion.Frontiers in bioengineering and biotechnology · 2023Article
- Assessing recovery rates of distinct exogenous controls for gDNA extraction efficiency using phenol-chloroform or silica-column based extractions.Journal of microbiological methods · 2022Article
- Increased Abundance of Achromobacter xylosoxidans and Bacillus cereus in Upper Airway Transcriptionally Active Microbiome of COVID-19 Mortality Patients Indicates Role of Co-Infections in Disease Severity and Outcome.Microbiology spectrum · 2022Article
- Pseudomonas aeruginosa modulates neutrophil granule exocytosis in an in vitro model of airway infection.Immunology and cell biology · 2022Article
- Exploiting the Anti-Biofilm Effect of the Engineered Phage Endolysin PM-477 to Disrupt In Vitro Single- and Dual-Species Biofilms of Vaginal Pathogens Associated with Bacterial Vaginosis.Antibiotics (Basel, Switzerland) · 2022Article
- Six Bacterial Vaginosis-Associated Species Can Form anFrontiers in cellular and infection microbiology · 2022Article
- Antibiotics reduce bacterial load inAccess microbiology · 2022Article
- SARS-CoV-2, other respiratory viruses and bacteria in aerosols: Report from Kuwait's hospitals.Indoor air · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While considerable research has focused on studying individual-species, we now face the challenge of determining how interspecies interactions alter bacterial behaviours and pathogenesis. Pseudomonas aeruginosa and Staphylococcus aureus are often found to co-infect cystic-fibrosis patients. Curiously, their interaction is reported as competitive under laboratory conditions. Selecting appropriate methodologies is therefore critical to analyse multi-species communities. Herein, we demonstrated the major biases associated with qPCR quantification of bacterial populations and optimized a RNA-based qPCR able not only to quantify but also to characterize microbial interactions within dual-species biofilms composed by P. aeruginosa and S. aureus, as assessed by gene expression quantification. qPCR quantification was compared with flow-cytometry and culture-based quantification. Discrepancies between culture independent and culture dependent methods could be the result of the presence of viable but not-cultivable bacteria within the biofilm. Fluorescence microscopy confirmed this. A higher sensitivity to detect viable cells further highlights the potentialities of qPCR approach to quantify biofilm communities. By using bacterial RNA and an exogenous mRNA control, it was also possible to characterize bacterial transcriptomic profile, being this a major advantage of this method.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.