ArticleNature communications2019
Nanomechanics and co-transcriptional folding of Spinach and Mango.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Rolling circle transcription-based fluorescent aptasensor for highly sensitive and onsite detection of microcystin-LR.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Article
- High-throughput, fluorescent-aptamer-based measurements of steady-state transcription rates for the Mycobacterium tuberculosis RNA polymerase.Nucleic acids research · 2023Article
- High-throughput, fluorescent-aptamer-based measurements of steady-state transcription rates forbioRxiv : the preprint server for biology · 2023Article
- Single Molecule FRET Analysis of CRISPR Cas9 Single Guide RNA Folding Dynamics.The journal of physical chemistry. B · 2023Article
- DNA-RNA hybrid G-quadruplex tends to form near the 3' end of telomere overhang.Biophysical journal · 2022Article
- Programming cell-free biosensors with DNA strand displacement circuits.Nature chemical biology · 2022Article
- Toehold-mediated strand displacement to measure released product from self-cleaving ribozymes.RNA (New York, N.Y.) · 2022Article
- Development of the DNA-based biosensors for high performance in detection of molecular biomarkers: More rapid, sensitive, and universal.Biosensors & bioelectronics · 2022Review
- Rhodamine 6G-Ligand Influencing G-Quadruplex Stability and Topology.International journal of molecular sciences · 2021Article
- Intracellular mRNA transport and localized translation.Nature reviews. Molecular cell biology · 2021Review
- Broad Applications of Thiazole Orange in Fluorescent Sensing of Biomolecules and Ions.Molecules (Basel, Switzerland) · 2021Review
- Photophysics of DFHBI bound to RNA aptamer Baby Spinach.Scientific reports · 2021Article
- Real-time monitoring of single ZTP riboswitches reveals a complex and kinetically controlled decision landscape.Nature communications · 2020Article
- Streamlining effects of extra telomeric repeat on telomeric DNA folding revealed by fluorescence-force spectroscopy.Nucleic acids research · 2019Article
- Understanding spatiotemporal coupling of gene expression using single molecule RNA imaging technologies.TranscriptionArticle
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2 authors.
Funding
Abstract
Recent advances in fluorogen-binding "light-up" RNA aptamers have enabled protein-free detection of RNA in cells. Detailed biophysical characterization of folding of G-Quadruplex (GQ)-based light-up aptamers such as Spinach, Mango and Corn is still lacking despite the potential implications on their folding and function. In this work we employ single-molecule fluorescence-force spectroscopy to examine mechanical responses of Spinach2, iMangoIII and MangoIV. Spinach2 unfolds in four discrete steps as force is increased to 7 pN and refolds in reciprocal steps upon force relaxation. In contrast, GQ-core unfolding in iMangoIII and MangoIV occurs in one discrete step at forces >10 pN and refolding occurred at lower forces showing hysteresis. Co-transcriptional folding using superhelicases shows reduced misfolding propensity and allowed a folding pathway different from refolding. Under physiologically relevant pico-Newton levels of force, these aptamers may unfold in vivo and subsequently misfold. Understanding of the dynamics of RNA aptamers will aid engineering of improved fluorogenic modules for cellular applications.
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