Evidence map›Paper›PMID 31540829›Full record

ArticleCell host & microbe2019

Systematic Identification of Host Cell Regulators of Legionella pneumophila Pathogenesis Using a Genome-wide CRISPR Screen.

Edwin E Jeng, Varun Bhadkamkar, Nnejiuwa U Ibe, Haley Gause, Lihua Jiang, Joanne Chan, Ruiqi Jian, David Jimenez-Morales, Erica Stevenson, Nevan J Krogan and 4 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell host & microbe, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.

0numbers the graph read from it
0cells of the map it votes in
62citing papers in PubMed
8.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

62 citing papers in PubMed, 94 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Bltp1 Is Required for Survival and Normal Development and Function of the Neuromuscular Junction in Mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025
    Article
  5. Article
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  7. Structural clues about bridge-mediated lipid transfer.Nature structural & molecular biology · 2025
    Article
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  11. Article
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  14. Article
  15. Review
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  20. Article

2 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 1 country.

Edwin E JengDepartment of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA; Program in Cancer Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Varun BhadkamkarDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA.
Nnejiuwa U IbeDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA.
Haley GauseDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA.
Lihua JiangDepartment of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA.
Joanne ChanDepartment of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA.
Ruiqi JianDepartment of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA.
David Jimenez-MoralesDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA; Quantitative Biosciences Institute (QBI), University of California, San Francisco, San Francisco, CA, USA; The J. David Gladstone Institutes, San Francisco, CA, USA.
Erica StevensonDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA; Quantitative Biosciences Institute (QBI), University of California, San Francisco, San Francisco, CA, USA; The J. David Gladstone Institutes, San Francisco, CA, USA.
Nevan J KroganDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA; Quantitative Biosciences Institute (QBI), University of California, San Francisco, San Francisco, CA, USA; The J. David Gladstone Institutes, San Francisco, CA, USA.
Danielle L SwaneyDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA; Quantitative Biosciences Institute (QBI), University of California, San Francisco, San Francisco, CA, USA; The J. David Gladstone Institutes, San Francisco, CA, USA.
Michael P SnyderDepartment of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA.
Shaeri MukherjeeDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA. Electronic address: shaeri.mukherjee@ucsf.edu.
Michael C BassikDepartment of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address: bassik@stanford.edu.
Stanford University · USUniversity of California, San Francisco · USQuantitative BioSciences · USGladstone Institutes · US

Funding

RESEARCH PROJECT 2U19AI135990 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Nevan J Krogan · 2018 to 2026
$21.4M
Leveraging innovative technologies in basic and clinical cancer researchT32CA009302 · NCI · STANFORD UNIVERSITY · PI LAURA D ATTARDI, Aaron M Newman · 1985 to 2026
$19.1M
GENETICS OF EUCARYOTIC AND PROCARYOTIC ORGANISMST32GM007810 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ASHRAFI, KAVEH, NARLIKAR, GEETA J · 1985 to 2020
$15.1M
Accelerating drug development and repurposing using systematic genetic interactioDP2HD084069 · NICHD · STANFORD UNIVERSITY · PI BASSIK, MICHAEL C · 2014 to 2014
$2.4M
Subversion of membrane transport pathways by Legionella pneumophilaR01AI118974 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MUKHERJEE, SHAERI · 2016 to 2020
$2.0M
NCI NIH HHS T32 CA009302NIAID NIH HHS R01 AI118974NIAID NIH HHS U19 AI135990NICHD NIH HHS DP2 HD084069
6 · The paper itself

Abstract

During infection, Legionella pneumophila translocates over 300 effector proteins into the host cytosol, allowing the pathogen to establish an endoplasmic reticulum (ER)-like Legionella-containing vacuole (LCV) that supports bacterial replication. Here, we perform a genome-wide CRISPR-Cas9 screen and secondary targeted screens in U937 human monocyte/macrophage-like cells to systematically identify host factors that regulate killing by L. pneumophila. The screens reveal known host factors hijacked by L. pneumophila, as well as genes spanning diverse trafficking and signaling pathways previously not linked to L. pneumophila pathogenesis. We further characterize C1orf43 and KIAA1109 as regulators of phagocytosis and show that RAB10 and its chaperone RABIF are required for optimal L. pneumophila replication and ER recruitment to the LCV. Finally, we show that Rab10 protein is recruited to the LCV and ubiquitinated by the effectors SidC/SdcA. Collectively, our results provide a wealth of previously undescribed insights into L. pneumophila pathogenesis and mammalian cell function.

Indexed as

AnimalsBacterial ProteinsCell LineClustered Regularly Interspaced Short Palindromic RepeatsEndoplasmic ReticulumGuanine Nucleotide Exchange FactorsHEK293 CellsHeLa CellsHumansLegionella pneumophilaLegionnaires' DiseaseMacrophagesMembrane ProteinsMicePhagocytosisProteinsBacterial ProteinsC1orf43 protein, humanGuanine Nucleotide Exchange FactorsKIAA1109 protein, humanMembrane ProteinsProteinsRab10 protein, humanrab GTP-Binding ProteinsRABIF protein, humanSidC protein, Legionella pneumophilaVirulence FactorsC1ORF43CRISPR screenhost-pathogen interactionintracellular bacteriaLegionella pneumophilapathogenphagocytosisRAB10

Identifiers

PMID31540829
PMCPMC6800164
OpenAlexW2974855504

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.