ArticleCartilage2021
Optimization of Protocol for Isolation of Chondrocytes from Human Articular Cartilage.
Article in Cartilage, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The trial behind it
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Elevated DNA 5-hydroxymethylation promotes chondrocyte differentiation via TET-mediated epigenetic downregulation of hTERT in human mesenchymal stem cells.Histochemistry and cell biology · 2026Article
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- Specialized pro-resolving mediators alleviate inflammation and cartilage breakdown in vitro and may play a pivotal role in platelet-rich plasma.Scientific reports · 2025Article
- Curcumin monoglucuronide protects cartilage from progressive degeneration by blocking MMP-13.Archives of pharmacal research · 2025Article
- The phenotypic characterization of mouse floxedRegenerative therapy · 2025Article
- Isolation and Culturing of Newborn Rat Nasal Chondrocytes.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Arctiin alleviates knee osteoarthritis by suppressing chondrocyte oxidative stress induced by accumulated iron via AKT/NRF2/HO-1 signaling pathway.Scientific reports · 2024Article
- 3D-printed constructs deliver bioactive cargos to expedite cartilage regeneration.Journal of pharmaceutical analysis · 2024Review
- Insufficiency of collagenases in establishment of primary chondrocyte culture from cartilage of elderly patients receiving total joint replacement.Cell and tissue banking · 2023Article
- Chondroprotective Effects ofPreventive nutrition and food science · 2023Article
- Towards Clinical Translation of In Situ Cartilage Engineering Strategies: Optimizing the Critical Facets of a Cell-Laden Hydrogel Therapy.Tissue engineering and regenerative medicine · 2023Article
- Article
- The Endplate Role in Degenerative Disc Disease Research: The Isolation of Human Chondrocytes from Vertebral Endplate-An Optimised Protocol.Bioengineering (Basel, Switzerland) · 2022Article
- Article
- 3D Printing for Bone-Cartilage Interface Regeneration.Frontiers in bioengineering and biotechnology · 2022Review
- Article
- Establishing SW1353 Chondrocytes as a Cellular Model of Chondrolysis.Life (Basel, Switzerland) · 2021Article
- Protective effects of stem cells from human exfoliated deciduous teeth derived conditioned medium on osteoarthritic chondrocytes.PloS one · 2020Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveCartilage tissue engineering has evolved as one of the therapeutic strategies for cartilage defect, which relies on a large number of viable chondrocytes. Because of limited availability of cartilage and low chondrocytes yield from cartilage, the need for an improve isolation protocol for maximum yield of viable cells is a key to achieving successful clinical constructs. This study optimizes and compares different protocols for isolation of chondrocytes from cartilage.
designWe employed enzymatic digestion of cartilage using collagenase II and trypsin. The chondrocytes yield, growth kinetics, aggrecan, and collagen type 2 (COL2) expression were evaluated. Collagen type 1 (COL1) mRNA expression was assessed to monitor the possibility of chondrocytes dedifferentiation.
resultsChondrocyte yield per gram of cartilage was significantly higher (
conclusionsCollagenase in HBSS yield the highest number of viable chondrocytes and the isolated cells expressed chondrocyte phenotype. This protocol can be employed to generate large number of viable chondrocytes, particularly with limited cartilage biopsies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.