Evidence map›Paper›PMID 31540100›Full record

ArticleCells2019

The Anti-Apoptotic Effect of ASC-Exosomes in an In Vitro ALS Model and Their Proteomic Analysis.

Roberta Bonafede, Jessica Brandi, Marcello Manfredi, Ilaria Scambi, Lorenzo Schiaffino, Flavia Merigo, Ermanna Turano, Bruno Bonetti, Emilio Marengo, Daniela Cecconi and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.

0numbers the graph read from it
0cells of the map it votes in
57citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

57 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Roberta BonafedeDepartment of Neurological, Biomedical and Movement Science, University of Verona, 37134 Verona, Italy. roberta.bonafede@univr.it.
Jessica BrandiDepartment of Biotechnology, University of Verona, 37134 Verona, Italy. jessica.brandi@univr.it.
Marcello ManfrediDepartment of Translational Medicine, University of Piemonte Orientale, 28100 Novara, Italy. marcello.manfredi@uniupo.it.
Ilaria ScambiDepartment of Neurological, Biomedical and Movement Science, University of Verona, 37134 Verona, Italy. ilaria.scambi@univr.it.
Lorenzo SchiaffinoDepartment of Neurological, Biomedical and Movement Science, University of Verona, 37134 Verona, Italy. lorenzo.schiaffino@univr.it.
Flavia MerigoDepartment of Neurological, Biomedical and Movement Science, University of Verona, 37134 Verona, Italy. flavia.merigo@univr.it.
Ermanna TuranoDepartment of Neurological, Biomedical and Movement Science, University of Verona, 37134 Verona, Italy. ermanna.turano@univr.it.
Bruno BonettiNeurology Unit, Azienda Ospedaliera Universitaria Integrata Verona, 37126 Verona, Italy. bruno.bonetti@univr.it.
Emilio MarengoCenter for Translational Research on Autoimmune and Allergic Diseases, University of Piemonte Orientale, Italy. emilio.marengo@uniupo.it.
Daniela CecconiDepartment of Biotechnology, University of Verona, 37134 Verona, Italy. daniela.cecconi@univr.it.
Raffaella MariottiDepartment of Neurological, Biomedical and Movement Science, University of Verona, 37134 Verona, Italy. raffaella.mariotti@univr.it.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Stem cell therapy represents a promising approach in the treatment of several neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). The beneficial effect of stem cells is exerted by paracrine mediators, as exosomes, suggesting a possible potential use of these extracellular vesicles as non-cell based therapy. We demonstrated that exosomes isolated from adipose stem cells (ASC) display a neuroprotective role in an in vitro model of ALS. Moreover, the internalization of ASC-exosomes by the cells was shown and the molecules and the mechanisms by which exosomes could exert their beneficial effect were addressed. We performed for the first time a comprehensive proteomic analysis of exosomes derived from murine ASC. We identified a total of 189 proteins and the shotgun proteomics analysis revealed that the exosomal proteins are mainly involved in cell adhesion and negative regulation of the apoptotic process. We correlated the protein content to the anti-apoptotic effect of exosomes observing a downregulation of pro-apoptotic proteins Bax and cleaved caspase-3 and upregulation of anti-apoptotic protein Bcl-2 α, in an in vitro model of ALS after cell treatment with exosomes. Overall, this study shows the neuroprotective effect of ASC-exosomes after their internalization and their global protein profile, that could be useful to understand how exosomes act, demonstrating that they can be employed as therapy in neurodegenerative diseases.

Indexed as

ApoptosisModels, BiologicalProteomicsAdipocytesAnimalsCells, CulturedExosomesMiceMice, Inbred C57BLNeuroprotective AgentsStem CellsNeuroprotective Agentsamyotrophic lateral sclerosisapoptosisextracellular vesiclesmesenchymal stem cellsproteomic profilingtherapy

Identifiers

PMID31540100
PMCPMC6770878

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.