Evidence map›Paper›PMID 31539522›Full record

ArticleAnalytical biochemistry2019

Perfused murine heart optical transmission spectroscopy using optical catheter and integrating sphere: Effects of ischemia/reperfusion.

Tyler M Bauer, Abigail V Giles, Junhui Sun, Armel Femnou, Raul Covian, Elizabeth Murphy, Robert S Balaban

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical biochemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
1.0field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Sex differences in mitochondrial CaJournal of molecular and cellular cardiology · 2026
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  9. Mechanisms of reduced myocardial energetics of the dystrophic heart.American journal of physiology. Heart and circulatory physiology · 2024
    Article
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  12. Review
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  14. Mitochondrial permeability transition pore-dependent necrosis.Journal of molecular and cellular cardiology · 2023
    Review
  15. Article
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Tyler M BauerLaboratory of Cardiac Physiology, National Heart, Lung and Blood Institute, Bethesda, MD, USA. Electronic address: Tyler.Bauer@nih.gov.
Abigail V GilesLaboratory of Cardiac Energetics, National Heart, Lung and Blood Institute, Bethesda, MD, USA. Electronic address: Abigail.Giles@nih.gov.
Junhui SunLaboratory of Cardiac Physiology, National Heart, Lung and Blood Institute, Bethesda, MD, USA. Electronic address: Junhui.sun@nih.gov.
Armel FemnouLaboratory of Cardiac Energetics, National Heart, Lung and Blood Institute, Bethesda, MD, USA. Electronic address: Armel.Femnou@nih.gov.
Raul CovianLaboratory of Cardiac Energetics, National Heart, Lung and Blood Institute, Bethesda, MD, USA. Electronic address: Raul.coviangarcia@nih.gov.
Elizabeth MurphyLaboratory of Cardiac Physiology, National Heart, Lung and Blood Institute, Bethesda, MD, USA. Electronic address: muphy1@mail.nih.gov.
Robert S BalabanLaboratory of Cardiac Energetics, National Heart, Lung and Blood Institute, Bethesda, MD, USA. Electronic address: Balabanr@nhlbi.nih.gov.
National Heart Lung and Blood Institute · USEnergetics (United States) · US

Funding

CONTROL OF CELLULAR ENERGY METABOLISMZ01HL004601 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI BALABAN, ROBERT S · 1988 to 2008
$4.4M
Intramural NIH HHS Z01 HL004601
6 · The paper itself

Abstract

Tissue transmission optical absorption spectroscopy provides dynamic information on metabolism and function. Murine genetic malleability makes it a major model for heart research. The diminutive size of the mouse heart makes optical transmission studies challenging. Using a perfused murine heart center mounted in an integrating sphere for light collection with a ventricular cavity optical catheter as an internal light source provided an effective method of optical data collection in this model. This approach provided high signal to noise optical spectra which when fit with model spectra provided information on tissue oxygenation and redox state. This technique was applied to the study of cardiac ischemia and ischemia reperfusion which generates extreme heart motion, especially during the ischemic contracture. The integrating sphere reduced motion artifacts associated with a fixed optical pickup and methods were developed to compensate for changes in tissue thickness. During ischemia, rapid decreases in myoglobin oxygenation occurred along with increases in cytochrome reduction levels. Surprisingly, when ischemic contracture occurred, myoglobin remained fully deoxygenated, while the cytochromes became more reduced consistent with a further, and critical, reduction of mitochondrial oxygen tension during ischemic contraction. This optical arrangement is an effective method of monitoring murine heart metabolism.

Indexed as

Optical DevicesPerfusionAnimalsHeartHeparinInjections, IntraperitonealLeast-Squares AnalysisMiceMice, Inbred C57BLMicrospheresMitochondriaPentobarbitalReperfusion InjurySpectrum AnalysisHeparinPentobarbitalCytochromesLinear least squares fittingMitochondria membrane potentialMyoglobinOptical pathlengthOxidative phosphorylationOxygen

Identifiers

PMID31539522
PMCPMC6915838
OpenAlexW2973689346

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.