Evidence map›Paper›PMID 31539501›Full record

ArticleCell2019

Genetic and Mechanical Regulation of Intestinal Smooth Muscle Development.

Tyler R Huycke, Bess M Miller, Hasreet K Gill, Nandan L Nerurkar, David Sprinzak, L Mahadevan, Clifford J Tabin

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.

0numbers the graph read from it
0cells of the map it votes in
69citing papers in PubMed
6.5field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

69 citing papers in PubMed, 138 citations in OpenAlex.

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  13. Anisotropic stretch biases the self-organization of actin fibers in multicellular Hydra aggregates.Proceedings of the National Academy of Sciences of the United States of America · 2025
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9 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 2 countries.

Tyler R HuyckeDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA.
Bess M MillerDepartment of Orthopedic Surgery, Brigham and Women's Hospital, Boston, MA 02115, USA.
Hasreet K GillDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA.
Nandan L NerurkarDepartment of Biomedical Engineering, Columbia University, New York, NY 10027, USA.
David SprinzakDepartment of Biochemistry and Molecular Biology, George S. Wise Faculty of Life Science, Tel Aviv University, Tel Aviv, Israel.
L MahadevanSchool of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA; Department of Physics, Harvard University, Cambridge, MA 02138, USA; Kavli Institute for Bionano Science and Technology, Harvard University, Cambridge, MA 02138, USA.
Clifford J TabinDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA. Electronic address: tabin@genetics.med.harvard.edu.
Harvard University · USBrigham and Women's Hospital · USColumbia University · USTel Aviv University · IL

Funding

STRUCTURE-FUNCTION RELATIONSHIPS IN THE ALIMENTARY TRACTP30DK034854 · NIDDK · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI JONATHAN C KAGAN · 1986 to 2026
$32.4M
Signals and mechanical forces controlling radial gut morphogenesisR01HD087234 · NICHD · HARVARD MEDICAL SCHOOL · PI TABIN, CLIFFORD J. · 2016 to 2025
$3.2M
Radial patterning of smooth muscle in the vertebrate gutF31DK111059 · NIDDK · HARVARD MEDICAL SCHOOL · PI HUYCKE, TYLER · 2016 to 2017
$63k
NICHD NIH HHS R01 HD087234NIDDK NIH HHS F31 DK111059NIDDK NIH HHS P30 DK034854
6 · The paper itself

Abstract

The gastrointestinal tract is enveloped by concentric and orthogonally aligned layers of smooth muscle; however, an understanding of the mechanisms by which these muscles become patterned and aligned in the embryo has been lacking. We find that Hedgehog acts through Bmp to delineate the position of the circumferentially oriented inner muscle layer, whereas localized Bmp inhibition is critical for allowing formation of the later-forming, longitudinally oriented outer layer. Because the layers form at different developmental stages, the muscle cells are exposed to unique mechanical stimuli that direct their alignments. Differential growth within the early gut tube generates residual strains that orient the first layer circumferentially, and when formed, the spontaneous contractions of this layer align the second layer longitudinally. Our data link morphogen-based patterning to mechanically controlled smooth muscle cell alignment and provide a mechanistic context for potentially understanding smooth muscle organization in a wide variety of tubular organs.

Indexed as

AnimalsBody PatterningBone Morphogenetic ProteinsCell DifferentiationChick EmbryoEmbryo, MammalianFemaleGene Expression Regulation, DevelopmentalHedgehog ProteinsIntestinal MucosaMaleMiceMice, Inbred C57BLMice, TransgenicMuscle DevelopmentMuscle, SmoothBone Morphogenetic ProteinsHedgehog ProteinsBmpcell orientationdifferentiationgut developmentHedgehogmechanical forcesmorphogenesispatterningsmooth muscle

Identifiers

PMID31539501
PMCPMC6756183
OpenAlexW2974514979

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.