ArticleCell2019
Rapid Generation of Somatic Mouse Mosaics with Locus-Specific, Stably Integrated Transgenic Elements.
Open access · greenAbstract read
In one paragraph
Article in Cell, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
0numbers the graph read from it
0cells of the map it votes in
51citing papers in PubMed
2.2field-weighted citation impact, top 11% of its field
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
51 citing papers in PubMed, 72 citations in OpenAlex.
- Levetiracetam therapeutically targets GABAergic synapses in diffuse midline glioma.Nature medicine · 2026Article
- Cell autonomous regional differences in oligodendrocyte lineage development and responses to oncohistone H3.3 K27M.Nature communications · 2026Article
- Article
- Review
- Peptide codes for organ-selective mRNA delivery.Nature materials · 2026Article
- A portable, ultra-low cost, open-source, pedal-controlled microinjector for laboratory use.PloS one · 2026Article
- Dysregulated Microglial Synaptic Engulfment in Diffuse Midline Glioma.bioRxiv : the preprint server for biology · 2025Article
- Serotonergic neuron-glioma interactions drive high-grade glioma pathophysiology.bioRxiv : the preprint server for biology · 2025Article
- Olig1/2 Orchestrates Progenitor Cell Fates during Mammalian Cortical Gliogenesis and Gliomagenesis.Nature communications · 2025Article
- Cholinergic neuronal activity promotes diffuse midline glioma growth through muscarinic signaling.Cell · 2025Article
- A parallelly distributed microscope and software system for scalable high-throughput multispectral 3D imaging.bioRxiv : the preprint server for biology · 2025Article
- CoSiDeX: A hyperspectral fluorescent protein resource for highly multiplexed imaging.bioRxiv : the preprint server for biology · 2025Article
- Precision preclinical modeling to advance cancer treatment.Journal of the National Cancer Institute · 2025Review
- Establishment of a mouse organ culture model of fetal cleft lip for the evaluation of adipose-derived stem cell therapy.Regenerative therapy · 2025Article
- Commensal papillomavirus immunity preserves the homeostasis of highly mutated normal skin.Cancer cell · 2025Article
- Zebrafish models in glioma research: advances in methodologies, mechanistic insights, and therapeutic frontiers.Frontiers in immunology · 2025Review
- In vivo mouse models for adult brain tumors: Exploring tumorigenesis and advancing immunotherapy development.Neuro-oncology · 2024Review
- Review
- Review
- Red2Flpe-SCON: a versatile, multicolor strategy for generating mosaic conditional knockout mice.Nature communications · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
29 authors at 5 institutions in 1 country.
Gi Bum KimBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
David Rincon Fernandez PachecoBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
David SaxonBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Amy YangBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Sara SabetBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Marina Dutra-ClarkeBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Rachelle LevyBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Ashley WatkinsBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Hannah ParkBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Aslam Abbasi AkhtarBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Paul W LineschBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Naomi KobritzBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Swasty S ChandraBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Katie GrausamBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Alberto Ayala-SarmientoBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Jessica MolinaBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Kristyna SedivakovaBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Kendy HoangDepartment of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz, 1156 High St., Santa Cruz, CA 95064, USA.
Jeremiah TsyporinDepartment of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz, 1156 High St., Santa Cruz, CA 95064, USA.
Daniel S GareauLaboratory for Investigative Dermatology, The Rockefeller University, New York, NY 10065, USA.
Mariella G FilbinDepartment of Pediatric Oncology, Dana-Farber Boston Children's Cancer and Blood Disorders Center, Boston, MA 02215, USA; Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
Serguei BannykhDepartment of Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Chintda SantiskulvongCenter for Neural Sciences in Medicine, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Yizhou WangCenter for Neural Sciences in Medicine, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Jie TangCenter for Neural Sciences in Medicine, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Mario L SuvaDepartment of Pathology and Center for Cancer Research, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA; Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
Bin ChenDepartment of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz, 1156 High St., Santa Cruz, CA 95064, USA.
Moise DanielpourBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Department of Neurosurgery, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Joshua J BreunigBoard of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Center for Neural Sciences in Medicine, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address: joshua.breunig@cshs.org.
Cedars-Sinai Medical Center · USUniversity of California, Santa Cruz · USDana-Farber/Boston Children's Cancer and Blood Disorders Center · USHarvard University · USRockefeller University · US
Funding
Lineage Progression of Cortical Neural Stem CellsR01NS089777 · NINDS · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI CHEN, BIN · 2015 to 2025
$4.7MIntegrated Interdisciplinary, inter-university PhD Program Computational Biology (T32)T32EB009403 · NIBIB · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI BAHAR, IVET, BAR-JOSEPH, ZIV · 2009 to 2023
$4.4MTranscriptional regulation of neuronal identity and connectivityR01MH094589 · NIMH · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI CHEN, BIN · 2011 to 2021
$3.7MA Genetic Toolbox for the Identification and Interrogation of Tumor Cells of Origin, Propagation, and Recurrence From Patient-derived Oncogenic DriversR33CA202900 · NCI · CEDARS-SINAI MEDICAL CENTER · PI BREUNIG, JOSHUA JOHN · 2016 to 2018
$1.2MGESTALT Barcoding and Single-cell Transcriptomics of Tumor Cell Evolution in Personalized Tumor ModelsR33CA236687 · NCI · CEDARS-SINAI MEDICAL CENTER · PI BREUNIG, JOSHUA JOHN · 2019 to 2021
$1.2MGraduate Program in Biomedical Sciences and Translational MedicineT32GM118288 · NIGMS · CEDARS-SINAI MEDICAL CENTER · PI PARKS, WILLIAM C · 2017 to 2021
$758kDirect Differentiations of iPSCs: an Autologous and Scalable Source of Striatal Medium Spiny Neurons for Cellular Transplantation TherapyR03NS101529 · NINDS · CEDARS-SINAI MEDICAL CENTER · PI BREUNIG, JOSHUA JOHN · 2017 to 2018
$175kNCI NIH HHS R33 CA202900NCI NIH HHS R33 CA236687NIBIB NIH HHS T32 EB009403NIGMS NIH HHS T32 GM118288NIMH NIH HHS R01 MH094589NINDS NIH HHS R01 NS089777NINDS NIH HHS R03 NS101529
Abstract
In situ transgenesis methods such as viruses and electroporation can rapidly create somatic transgenic mice but lack control over copy number, zygosity, and locus specificity. Here we establish mosaic analysis by dual recombinase-mediated cassette exchange (MADR), which permits stable labeling of mutant cells expressing transgenic elements from precisely defined chromosomal loci. We provide a toolkit of MADR elements for combination labeling, inducible and reversible transgene manipulation, VCre recombinase expression, and transgenesis of human cells. Further, we demonstrate the versatility of MADR by creating glioma models with mixed reporter-identified zygosity or with "personalized" driver mutations from pediatric glioma. MADR is extensible to thousands of existing mouse lines, providing a flexible platform to democratize the generation of somatic mosaic mice. VIDEO ABSTRACT.
Indexed as
Disease Models, AnimalAnimalsBrain NeoplasmsCell Line, TumorFemaleGene TargetingGenetic LociGliomaHEK293 CellsHumansMaleMiceMice, Inbred C57BLMice, TransgenicMutagenesis, InsertionalNeural Stem CellsRecombinasesAAVS1 locusBrainbowCRISPR/Cas9 base editorsependymomaepigeneticsMADR MAXRMCEscATAC-seqscRNA-seqVCre
Identifiers
What OpenQuestion holds
Textmetadata
LicenceTDM
Read underepoch 390
Registered trials
None linked
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.