ArticlePloS one2019
Bulked segregant analysis RNA-seq (BSR-Seq) validated a stem resistance locus in Aegilops umbellulata, a wild relative of wheat.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 37 citations in OpenAlex.
- Genetic analyses of leaf traits in an interspecific Zoysia japonica × Zoysia matrella FThe plant genome · 2026Article
- Genomes of Aegilops umbellulata provide new insights into unique structural variations and genetic diversity in the U-genome for wheat improvement.Plant biotechnology journal · 2024Article
- Chromosome stability of synthetic Triticum turgidum-Aegilops umbellulata hybrids.BMC plant biology · 2024Article
- Identification of a candidate gene for the I locus determining the dominant white bulb color in onion (Allium cepa L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Application of bulk segregant RNA-Seq (BSR-Seq) and allele-specific primers to study soybean powdery mildew resistance.BMC plant biology · 2024Article
- Chromosome-scale assembly of the wild wheat relative Aegilops umbellulata.Scientific data · 2023Article
- Suppression of ZEAXANTHIN EPOXIDASE 1 restricts stripe rust growth in wheat.Plant communications · 2023Article
- Transcript profiling of hairy vetch (Vicia villosa Roth) identified interesting genes for seed dormancy.The plant genome · 2023Article
- Conjunctive Analyses of BSA-Seq and BSR-Seq to Identify Candidate Genes Controlling the Black Lemma and Pericarp Trait in Barley.International journal of molecular sciences · 2023Article
- Screening of Candidate Genes Associated with Brown Stripe Resistance in Sugarcane via BSR-seq Analysis.International journal of molecular sciences · 2022Article
- Bulk segregant linkage mapping for rodent and human malaria parasites.Parasitology international · 2022Review
- Harnessing the potential of bulk segregant analysis sequencing and its related approaches in crop breeding.Frontiers in genetics · 2022Review
- SNP Discovery Using BSR-Seq Approach for Spot Blotch Resistance in Wheat (Frontiers in genetics · 2022Article
- RNA-Seq-based DNA marker analysis of the genetics and molecular evolution of Triticeae species.Functional & integrative genomics · 2021Review
- Dissection of Genetic Basis Underpinning Kernel Weight-Related Traits in Common Wheat.Plants (Basel, Switzerland) · 2021Article
- Article
- Comparative Transcriptomics and RNA-Seq-Based Bulked Segregant Analysis Reveals Genomic Basis UnderlyingFrontiers in microbiology · 2021Article
- Identification of Solanum Immune Receptors by Bulked Segregant RNA-Seq and High-Throughput Recombinant Screening.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Next Generation Sequencing Based Forward Genetic Approaches for Identification and Mapping of Causal Mutations in Crop Plants: A Comprehensive Review.Plants (Basel, Switzerland) · 2020Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many disease resistance genes that have been transferred from wild relatives to cultivated wheat have played a significant role in wheat production worldwide. Ae. umbellulata is one of the species within the genus Aegilops that have been successfully used as sources of resistance genes to leaf rust, stem rust and powdery mildew. The objectives of the current work was to validate the map position of a major QTL that confers resistance to the stem rust pathogen races Ug99 (TTKSK) and TTTTF with an independent bi-parental mapping population and to refine the QTL region with a bulk segregant analysis approach. Two F2 bi-parental mapping populations were developed from stem rust resistant Ae. umbellulata accessions (PI 298905 and PI 5422375) and stem rust susceptible accessions (PI 542369 and PI 554395). Firstly, one of the two populations was used to map the chromosome location of the resistance gene. Later on, the 2nd population was used to validate the chromosome location in combination with a bulk segregant analysis approach. For the bulk segregant analysis, RNA was extracted from a bulk of leaf tissues of 12 homozygous resistant F3 families, and a separate bulk of 11 susceptible homozygous F3 families derived from the PI 5422375 and PI 554395 cross. The RNA samples of the two bulks and the two parents were sequenced for SNPs identification. Stem rust resistance QTL was validated on chromosome 2U of Ae. umbellulata in the same region in both populations. With bulk segregant analysis, the QTL position was delimited within 3.2 Mbp. Although there were a large number of genes in the orthologous region of the detected QTL on chromosome 2D of Ae. tauschii, we detected only two Ae. umbellulata NLR genes which can be considered as a potential candidate genes.
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