ArticleGenome biology and evolution2019
A High-Resolution View of Adaptive Event Dynamics in a Plasmid.
Article in Genome biology and evolution, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Evaluating open LLMs for agentic analysis orchestration in a typical biomedical lab.bioRxiv : the preprint server for biology · 2026Article
- Detection of mutations: from Ames test to duplex sequencing.Frontiers in molecular biosciences · 2026Review
- The role of plasmid copy number and mutation rate in evolutionary outcomes.Nature ecology & evolution · 2025Article
- A mathematician's guide to plasmids: an introduction to plasmid biology for modellers.Microbiology (Reading, England) · 2023Review
- Plasmid Costs Explain Plasmid Maintenance, Irrespective of the Nature of Compensatory Mutations.Antibiotics (Basel, Switzerland) · 2023Article
- Conversion ofMicroorganisms · 2023Article
- Article
- Concerning Increase in Antimicrobial Resistance Patterns of Pathogenic Strains ofAntibiotics (Basel, Switzerland) · 2022Article
- Beyond horizontal gene transfer: the role of plasmids in bacterial evolution.Nature reviews. Microbiology · 2021Review
- Freely accessible ready to use global infrastructure for SARS-CoV-2 monitoring.bioRxiv : the preprint server for biology · 2021Article
- Mathematical Models of Plasmid Population Dynamics.Frontiers in microbiology · 2021Review
- Family reunion via error correction: an efficient analysis of duplex sequencing data.BMC bioinformatics · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Coadaptation between bacterial hosts and plasmids frequently results in adaptive changes restricted exclusively to host genome leaving plasmids unchanged. To better understand this remarkable stability, we transformed naïve Escherichia coli cells with a plasmid carrying an antibiotic-resistance gene and forced them to adapt in a turbidostat environment. We then drew population samples at regular intervals and subjected them to duplex sequencing-a technique specifically designed for identification of low-frequency mutations. Variants at ten sites implicated in plasmid copy number control emerged almost immediately, tracked consistently across the experiment's time points, and faded below detectable frequencies toward the end. This variation crash coincided with the emergence of mutations on the host chromosome. Mathematical modeling of trajectories for adaptive changes affecting plasmid copy number showed that such mutations cannot readily fix or even reach appreciable frequencies. We conclude that there is a strong selection against alterations of copy number even if it can provide a degree of growth advantage. This incentive is likely rooted in the complex interplay between mutated and wild-type plasmids constrained within a single cell and underscores the importance of understanding of intracellular plasmid variability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.