ArticleLab on a chip2019
Microfluidic rapid and autonomous analytical device (microRAAD) to detect HIV from whole blood samples.
Article in Lab on a chip, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed.
- A smartphone-based particle diffusometry platform for sub-attomolar detection of Vibrio cholerae in environmental water.Biosensors & bioelectronics · 2020Trial
- Molecular Diagnostics of Infectious Diseases.Yonago acta medica · 2026Review
- Antinuclear Antibodies Predict Treatment Escalation and Biologic Switching in Rheumatoid Arthritis.Diagnostics (Basel, Switzerland) · 2026Article
- Extraction-Free Scalable Sample Preparation for the Rapid Detection of HIV in Blood Plasma.Analytical chemistry · 2026Article
- Heat-actuated valve implementation in a point-of-care, paper-based microfluidic device for infectious disease detection.PloS one · 2026Article
- Rapid simultaneous self-testing of HIV and HCV viral loads with integrated RNA extraction and multiplex RT-PCR in under 1 hour.Biosensors & bioelectronics · 2025Article
- Extraction-free RT-RPA assay for detection of HPV16, HPV18, and HPV45 mRNA expression.Scientific reports · 2025Article
- A new approach for the detection of genetic alterations utilizing modified loop-mediated isothermal amplification reaction (LAMP).Scientific reports · 2025Article
- Compact multiplex PCR device for HIV-1 and HIV-2 viral load determination from finger-prick whole blood in resource-limited settings.Biosensors & bioelectronics · 2025Article
- Development of a Highly Multiplexed RT-LAMP Assay for Coverage of Genetic Sequence Diversity.Analytical chemistry · 2025Article
- Stakeholder considerations on acceptability and implementation of a novel rapid test for acute HIV infection: A qualitative study in Indiana.PLOS global public health · 2025Article
- Isothermal nucleic acid amplification for monitoring hand-foot-and-mouth disease: current status and future implications.Mikrochimica acta · 2024Review
- Rapid and portable quantification of HIV RNA via a smartphone-enabled digital CRISPR device and deep learning.Sensors and actuators reports · 2024Article
- A handheld HIV detection platform using paper-based sample preparation and real-time isothermal amplification.Microsystems & nanoengineering · 2024Article
- Real-Time Visualization of HIV-1 RNA Detection Using Loop-Mediated Isothermal Amplification-Enabled Particle Diffusometry.ACS sensors · 2024Article
- Sample preparation and detection methods in point-of-care devices towards future at-home testing.Lab on a chip · 2024Review
- Stakeholder-engaged development of a rapid test for detection of acute HIV infection.Research square · 2024Article
- Development of an integrated sample amplification control for salivary point-of-care pathogen testing.Analytica chimica acta · 2024Article
- Compact Point-of-Care Device for Self-Administered HIV Viral Load Tests from Whole Blood.ACS sensors · 2023Article
- A novel tailed primer nucleic acid test for detection of HPV 16, 18 and 45 DNA at the point of care.Scientific reports · 2023Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
While identifying acute HIV infection is critical to providing prompt treatment to HIV-positive individuals and preventing transmission, existing laboratory-based testing methods are too complex to perform at the point of care. Specifically, molecular techniques can detect HIV RNA within 8-10 days of transmission but require laboratory infrastructure for cold-chain reagent storage and extensive sample preparation performed by trained personnel. Here, we demonstrate our point-of-care microfluidic rapid and autonomous analysis device (microRAAD) that automatically detects HIV RNA from whole blood. Inside microRAAD, we incorporate vitrified amplification reagents, thermally-actuated valves for fluidic control, and a temperature control circuit for low-power heating. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) products are visualized using a lateral flow immunoassay (LFIA), resulting in an assay limit of detection of 100 HIV-1 RNA copies when performed as a standard tube reaction. Even after three weeks of room-temperature reagent storage, microRAAD automatically isolates the virus from whole blood, amplifies HIV-1 RNA, and transports amplification products to the internal LFIA, detecting as few as 3 × 105 HIV-1 viral particles, or 2.3 × 107 virus copies per mL of whole blood, within 90 minutes. This integrated microRAAD is a low-cost and portable platform to enable automated detection of HIV and other pathogens at the point of care.
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