ArticleScientific reports2019
Characterisation and localisation of the endocannabinoid system components in the adult human testis.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 79 citations in OpenAlex.
- Testicular histopathology and its association with germ cell numbers, serum concentrations of reproductive hormones, and semen quality.Andrology · 2026Article
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- Phytocannabinoids and Male Fertility: Implications ofPlants (Basel, Switzerland) · 2026Review
- CATSPERε extracellular domains are essential for sperm calcium channel assembly and activity modulation.Science advances · 2026Article
- From localization to function: comparative analysis of CB1 in sperm across species and its epigenetic role in humans.Cell death & disease · 2026Article
- Adolescent exposure to Δ9-tetrahydrocannabinol impairs testicular function in young adult male mice.Toxicological sciences : an official journal of the Society of Toxicology · 2025Article
- CATSPERε extracellular domains are essential for sperm calcium channel assembly and activity modulation.bioRxiv : the preprint server for biology · 2025Article
- CCDC89 is required for optimal sperm motility and male fertility in mammals.Human reproduction (Oxford, England) · 2025Article
- The major phytocannabinoids, delta-9-tetrahydrocannabinol (THC) and cannabidiol (CBD), affect the function of CatSper calcium channels in human sperm.Human reproduction (Oxford, England) · 2025Article
- The impact of cannabinoids on reproductive function.Reproduction (Cambridge, England) · 2025Review
- The Role of the Endocannabinoid System in Human Gametogenesis.International journal of molecular sciences · 2025Review
- Endocannabinoid system upregulates the enrichment and differentiation of human iPSC- derived spermatogonial stem cells via CB2R agonism.Biological research · 2025Article
- Identification of genes associated with testicular germ cell tumor susceptibility through a transcriptome-wide association study.American journal of human genetics · 2025Article
- Human sperm RNA in male infertility.Nature reviews. Urology · 2025Review
- Frailty detection with routine blood tests using data from the english longitudinal study of ageing (ELSA).European geriatric medicine · 2024Article
- MALDI Imaging Mass Spectrometry Reveals Lipid Alterations in Physiological and Sertoli Cell-Only Syndrome Human Testicular Tissue Sections.International journal of molecular sciences · 2024Article
- X‑chromosome loss rescues Sertoli cell maturation and spermatogenesis in Klinefelter syndrome.Cell death & disease · 2024Article
- The Acute Exposure of Human Adult Testis Tissue to Cannabinoids THC and CBD Does Not Impact Testosterone Production Nor Germ Cell Lineage.The world journal of men's health · 2023Article
- Expression of the endocannabinoid system and response to cannabinoid components by the human fetal testis.BMC medicine · 2023Article
- Developmental programming: Preconceptional and gestational exposure of sheep to a real-life environmental chemical mixture alters maternal metabolome in a fetal sex-specific manner.The Science of the total environment · 2023Article
Corrections and comments
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- Erratum issued
Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heavy use of cannabis (marijuana) has been associated with decreased semen quality, which may reflect disruption of the endocannabinoid system (ECS) in the male reproductive tract by exogenous cannabinoids. Components of ECS have been previously described in human spermatozoa and in the rodent testis but there is little information on the ECS expression within the human testis. In this study we characterised the main components of the ECS by immunohistochemistry (IHC) on archived testis tissue samples from 15 patients, and by in silico analysis of existing transcriptome datasets from testicular cell populations. The presence of 2-arachidonoylglycerol (2-AG) in the human testis was confirmed by matrix-assisted laser desorption ionization imaging analysis. Endocannabinoid-synthesising enzymes; diacylglycerol lipase (DAGL) and N-acyl-phosphatidylethanolamine-specific phospholipase D (NAPE-PLD), were detected in germ cells and somatic cells, respectively. The cannabinoid receptors, CNR1 and CNR2 were detected at a low level in post-meiotic germ cells and Leydig- and peritubular cells. Different transcripts encoding distinct receptor isoforms (CB1, CB1A, CB1B and CB2A) were also differentially distributed, mainly in germ cells. The cannabinoid-metabolising enzymes were abundantly present; the α/β-hydrolase domain-containing protein 2 (ABHD2) in all germ cell types, except early spermatocytes, the monoacylglycerol lipase (MGLL) in Sertoli cells, and the fatty acid amide hydrolase (FAAH) in late spermatocytes and post-meiotic germ cells. Our findings are consistent with a direct involvement of the ECS in regulation of human testicular physiology, including spermatogenesis and Leydig cell function. The study provides new evidence supporting observations that recreational cannabis can have possible deleterious effects on human testicular function.
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