ArticleeLife2019
Protein engineering expands the effector recognition profile of a rice NLR immune receptor.
Article in eLife, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed, 150 citations in OpenAlex.
- Harnessing Natural Diversity and Rational Design for Enhanced Plant Immunity.Plant, cell & environment · 2026Review
- Molecular Bases and Genetic Design of Rice Disease Resistance for Optimized Yield and Sustainable Agriculture.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Mechanisms and balanced regulation of plant immunity.Nature reviews. Molecular cell biology · 2026Review
- Engineering an Exo70 integrated domain of a barley NLR for improved blast resistance.The Plant cell · 2026Article
- Exploiting plant immune "switches" for resistance engineering.Stress biology · 2026Review
- Plant NLRs are getting into higher-order architectures.The Plant journal : for cell and molecular biology · 2026Review
- Mismatch screening in Nicotiana benthamiana to explore Pik-1/Pik-2 paired NLR platforms for receptor engineering.The New phytologist · 2026Article
- Unraveling plant immunity: from pathogen perception to resistance engineering.Science China. Life sciences · 2026Review
- Precision Editing of NLRS Improves Effector Recognition for Enhanced Disease Resistance.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- HIPPs of all trades: insights from pathogens on the function of HMA proteins at plasmodesmata.Journal of experimental botany · 2026Review
- TheProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- The resistance awakens: Diversity at the DNA, RNA, and protein levels informs engineering of plant immune receptors from Arabidopsis to crops.The Plant cell · 2025Review
- Structure-guided insights into the biology of fungal effectors.The New phytologist · 2025Review
- Helper NLR immune protein NRC3 evolved to evade inhibition by a cyst nematode virulence effector.PLoS genetics · 2025Article
- Resurrection of the Plant Immune Receptor Sr50 to Overcome Pathogen Immune Evasion.bioRxiv : the preprint server for biology · 2025Article
- Directed Evolution of a Plant Immune Receptor for Broad Spectrum Effector Recognition.bioRxiv : the preprint server for biology · 2025Article
- The role of Exo70s in plant defense against pathogens and insect pests and their application for crop breeding.Molecular breeding : new strategies in plant improvement · 2025Review
- All Roads Lead to Rome: Pathways to Engineering Disease Resistance in Plants.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Revisiting interactions between polygalacturonases and polygalacturonase-inhibiting proteins and their effects on plant health: a review.Frontiers in plant science · 2025Review
22 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Plant nucleotide binding, leucine-rich repeat (NLR) receptors detect pathogen effectors and initiate an immune response. Since their discovery, NLRs have been the focus of protein engineering to improve disease resistance. However, this approach has proven challenging, in part due to their narrow response specificity. Previously, we revealed the structural basis of pathogen recognition by the integrated heavy metal associated (HMA) domain of the rice NLR Pikp (Maqbool et al., 2015). Here, we used structure-guided engineering to expand the response profile of Pikp to variants of the rice blast pathogen effector AVR-Pik. A mutation located within an effector-binding interface of the integrated Pikp-HMA domain increased the binding affinity for AVR-Pik variants in vitro and in vivo. This translates to an expanded cell-death response to AVR-Pik variants previously unrecognized by Pikp in planta. The structures of the engineered Pikp-HMA in complex with AVR-Pik variants revealed the mechanism of expanded recognition. These results provide a proof-of-concept that protein engineering can improve the utility of plant NLR receptors where direct interaction between effectors and NLRs is established, particularly where this interaction occurs via integrated domains.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.