ArticleNano letters2019
Toward Absolute Molecular Numbers in DNA-PAINT.
Article in Nano letters, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Versatile and Scalable Reflective Micromirrors for Single-Objective Light Sheet Microscopy.Nano letters · 2026Article
- Stoichiometrically Defined Antibody-DNA Conjugates for Quantitative Super-Resolution Imaging.Nano letters · 2026Article
- Versatile and Scalable Reflective Micromirrors for Single-Objective Light Sheet Microscopy.bioRxiv : the preprint server for biology · 2026Article
- Impact of Docking Strand Design on Spatial Resolution in DNA-Points Accumulation for Imaging in Nanoscale Topography.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026Article
- A Simple, Ultrastable, and Cost-Effective Oxygen-Scavenging System for Long-Term DNA-PAINT Imaging.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Spatial Molecular Heterogeneity on Biofunctionalized Particles Quantified by Three-Dimensional Single-Molecule DNA-PAINT.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Cryosectioning-enhanced super-resolution microscopy for single-protein imaging across cells and tissues.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- DNA-PAINT Imaging with Hydrogel Imprinting and Clearing.ACS sensors · 2025Article
- Hybrid DNA Origami - Graphene Platform for Electrically-Gated Nanoscale Motion.Advanced materials interfaces · 2025Article
- A Bayesian Model to Count the Number of Two-State Emitters in a Diffraction Limited Spot.Nano letters · 2025Article
- Current capabilities and future perspectives of FCS: super-resolution microscopy, machine learning, and in vivo applications.Communications biology · 2023Review
- Shrinking gate fluorescence correlation spectroscopy yields equilibrium constants and separates photophysics from structural dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- A novel and robust method for counting components within bio-molecular complexes using fluorescence microscopy and statistical modelling.Scientific reports · 2022Article
- Systematic Assessment of the Accuracy of Subunit Counting in Biomolecular Complexes Using Automated Single-Molecule Brightness Analysis.The journal of physical chemistry letters · 2022Article
- Design Features to Accelerate the Higher-Order Assembly of DNA Origami on Membranes.The journal of physical chemistry. B · 2021Article
- Calibration-free counting of low molecular copy numbers in single DNA-PAINT localization clusters.Biophysical reports · 2021Article
- Completing the canvas: advances and challenges for DNA-PAINT super-resolution imaging.Trends in biochemical sciences · 2021Review
- Evaluating the effect of ionic strength on PNA:DNA duplex formation kinetics.RSC chemical biology · 2021Article
- Tracking single particles for hours via continuous DNA-mediated fluorophore exchange.Nature communications · 2021Article
- Single molecule imaging of protein aggregation in Dementia: Methods, insights and prospects.Neurobiology of disease · 2021Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-molecule localization microscopy (SMLM) has revolutionized optical microscopy, extending resolution down to the level of individual molecules. However, the actual counting of molecules relies on preliminary knowledge of the blinking behavior of individual targets or on a calibration to a reference. In particular for biological applications, great care has to be taken because a plethora of factors influence the quality and applicability of calibration-dependent approaches to count targets in localization clusters particularly in SMLM data obtained from heterogeneous samples. Here, we present localization-based fluorescence correlation spectroscopy (lbFCS) as the first absolute molecular counting approach for DNA-points accumulation for imaging in nanoscale topography (PAINT) microscopy and, to our knowledge, for SMLM in general. We demonstrate that lbFCS overcomes the limitation of previous DNA-PAINT counting and allows the quantification of target molecules independent of the localization cluster density. In accordance with the promising results of our systematic proof-of-principle study on DNA origami structures as idealized targets, lbFCS could potentially also provide quantitative access to more challenging biological targets featuring heterogeneous cluster sizes in the future.
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Registered trials
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