Evidence map›Paper›PMID 31534496›Full record

ArticleTheranostics2019

Flow cytometry-based FRET identifies binding intensities in PPARγ1 protein-protein interactions in living cells.

Verena Trümper, Andreas von Knethen, Annegret Preuß, Eugeny Ermilov, Steffen Hackbarth, Laura Kuchler, Sandra Gunne, Anne Schäfer, Tobias Bornhütter, György Vereb and 6 more

Open access · goldAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Theranostics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Receptor-Ligand Binding: Effect of Mechanical Factors.International journal of molecular sciences · 2023
    Review
  2. Review
  3. Redox Regulation of PPARPPAR research · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 5 institutions in 2 countries.

Verena TrümperInstitute of Biochemistry I - Pathobiochemistry, Faculty of Medicine, Goethe University Frankfurt, Theodor-Stern-Kai 7, 60590 Frankfurt/Main, Germany.
Andreas von KnethenInstitute of Biochemistry I - Pathobiochemistry, Faculty of Medicine, Goethe University Frankfurt, Theodor-Stern-Kai 7, 60590 Frankfurt/Main, Germany.
Annegret PreußDepartment of Physics, Humboldt University Berlin, Newtonstraße 15, 12489 Berlin, Germany.
Eugeny ErmilovDepartment of Physics, Humboldt University Berlin, Newtonstraße 15, 12489 Berlin, Germany.
Steffen HackbarthDepartment of Physics, Humboldt University Berlin, Newtonstraße 15, 12489 Berlin, Germany.
Laura KuchlerInstitute of Biochemistry I - Pathobiochemistry, Faculty of Medicine, Goethe University Frankfurt, Theodor-Stern-Kai 7, 60590 Frankfurt/Main, Germany.
Sandra GunneBranch for Translational Medicine and Pharmacology, Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Theodor-Stern-Kai 7, 60596 Frankfurt/Main, Germany.
Anne SchäferInstitute of Biochemistry I - Pathobiochemistry, Faculty of Medicine, Goethe University Frankfurt, Theodor-Stern-Kai 7, 60590 Frankfurt/Main, Germany.
Tobias BornhütterDepartment of Physics, Humboldt University Berlin, Newtonstraße 15, 12489 Berlin, Germany.
György VerebDepartment of Biophysics and Cell Biology, Faculty of Medicine, University of Debrecen, Egyetem tér 1, 4032 Debrecen, Hungary.
Lázló Ujlaky-NagyDepartment of Biophysics and Cell Biology, Faculty of Medicine, University of Debrecen, Egyetem tér 1, 4032 Debrecen, Hungary.
Bernhard BrüneInstitute of Biochemistry I - Pathobiochemistry, Faculty of Medicine, Goethe University Frankfurt, Theodor-Stern-Kai 7, 60590 Frankfurt/Main, Germany.
Beate RöderDepartment of Physics, Humboldt University Berlin, Newtonstraße 15, 12489 Berlin, Germany.
Michael SchindlerInstitute of Medical Virology and Epidemiology of Viral Diseases, University Hospital Tübingen, Karls University Tübingen, Elfriede-Aulhorn-Str. 6, 72076 Tübingen.
Michael J ParnhamBranch for Translational Medicine and Pharmacology, Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Theodor-Stern-Kai 7, 60596 Frankfurt/Main, Germany.
Tilo KnapeBranch for Translational Medicine and Pharmacology, Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Theodor-Stern-Kai 7, 60596 Frankfurt/Main, Germany.
Humboldt-Universität zu Berlin · DEFraunhofer Institute for Translational Medicine and Pharmacology · DEGoethe University Frankfurt · DEUniversity of Debrecen · HUFraunhofer Institute for Molecular Biology and Applied Ecology · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

PPARγ is a pharmacological target in inflammatory and metabolic diseases. Upon agonistic treatment or following antagonism, binding of co-factors is altered, which consequently affects PPARγ-dependent transactivation as well as its DNA-independent properties. Therefore, establishing techniques to characterize these interactions is an important issue in living cells.

Indexed as

AnimalsDimerizationFlow CytometryFluorescence Resonance Energy TransferHEK293 CellsHumansMiceNuclear Receptor Co-Repressor 1PPAR gammaProtein BindingProtein DomainsRetinoid X Receptor alphaNCOR1 protein, humanNuclear Receptor Co-Repressor 1PPAR gammaRetinoid X Receptor alphabinding affinity and intensityco-localization analysisflow cytometry-based FRET assayFRETN-CoR2NHR co-factors protein-protein interactionsPPARγ1RXRα

Identifiers

PMID31534496
PMCPMC6735382
OpenAlexW2968109464

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.