Evidence map›Paper›PMID 31533815›Full record

ArticleBMC research notes2019

Scalable methanol-free production of recombinant glucuronoyl esterase in Pichia pastoris.

C G Conacher, M P García-Aparicio, G Coetzee, W H van Zyl, J F Gӧrgens

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC research notes, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. The Model SystemLife (Basel, Switzerland) · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

C G ConacherDepartments of Process Engineering, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa.
M P García-AparicioDepartments of Process Engineering, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa. garcia@sun.ac.za.ORCID http://orcid.org/0000-0001-5312-6561
G CoetzeeDepartments of Process Engineering, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa.
W H van ZylDepartments of Microbiology, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa.
J F GӧrgensDepartments of Process Engineering, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa.
Stellenbosch University · ZA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveGlucuronoyl esterase (GE) is an emerging enzyme that improves fractionation of lignin-carbohydrate complexes. However, the commercial availability of GE is limited, which hinders the research of GE-based bioprocesses for its industrial application in lignocellulose biorefineries. This study evaluated a workable, cost-effective, and commercially scalable production strategy to improve the ease of GE-based research. This strategy consisted of a constitutive and methanol-free enzyme production step coupled with a two-step filtration process. The aim was to determine if this strategy can yield copious amounts of GE, by secretion into the extracellular medium with an acceptable purity that could allow its direct application. This approach was further validated for cellobiose dehydrogenase, another emerging lignocellulose degrading enzyme which is scarcely available at high cost.

resultsThe secreted recombinant enzymes were functionally produced in excess of levels previously reported for constitutive production (1489-2780 mg L

Indexed as

Batch Cell Culture TechniquesEsterasesExtracellular SpaceFermentationGlucuronic AcidMethanolPichiaRecombinant ProteinsEsterasesGlucuronic AcidMethanolRecombinant ProteinsCellobiose dehydrogenaseEnzyme bioprocess technologyGlucuronoyl esteraseLignocellulose biorefinery enzymesMethanol-free constitutive expression systemPichia pastoris

Identifiers

PMID31533815
PMCPMC6751620
OpenAlexW2973300356

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.