ArticleInternational journal of molecular sciences2019
Freezing-Thawing Procedures Remodel the Proteome of Ram Sperm before and after In Vitro Capacitation.
Article in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Small and Large Seminal Extracellular Vesicles Differentially Modulate Capacitation-Related Parameters in Frozen-Thawed Porcine Epididymal Spermatozoa.Reproduction in domestic animals = Zuchthygiene · 2026Article
- Impact of extended equilibration periods on in vitro post-thaw sperm quality in rams.Veterinary research communications · 2026Article
- Overcoming Batch Variability: A Chemically Defined XRS-Based System for Ovine In Vitro Embryo Production.Veterinary medicine international · 2026Article
- Effect of Capacitation on Proteomic Profile and Mitochondrial Parameters of Spermatozoa in Bulls.Journal of proteome research · 2025Article
- New Delivery Systems on Sperm Quality Improvement.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Inhibition of forward and reverse transport of CaBiological research · 2024Article
- Comparative Proteomic Identification of Ram Sperm before and after In Vitro Capacitation.Animals : an open access journal from MDPI · 2024Article
- Article
- A review on the functional roles of trehalose during cryopreservation of small ruminant semen.Frontiers in veterinary science · 2024Review
- TMT-Based Comparative Proteomic Analysis of the Spermatozoa of Buck (Capra hircus) and Ram (Ovis aries).Genes · 2023Article
- Ram Semen Cryopreservation for Portuguese Native Breeds: Season and Breed Effects on Semen Quality Variation.Animals : an open access journal from MDPI · 2023Article
- Testis-enriched ferlin, FER1L5, is required for CaScience advances · 2023Article
- The Cryopreserved Sperm Traits of Various Ram Breeds: Towards Biodiversity Conservation.Animals : an open access journal from MDPI · 2022Article
- Quantitative phosphoproteomics analyses reveal the regulatory mechanisms related to frozen-thawed sperm capacitation and acrosome reaction in yak (Frontiers in physiology · 2022Article
- Article
- Effect of insulin-like growth factor-1 complex of Simmental bull seminal plasma on post-thawed Kacang buck semen fertility.Veterinary world · 2021Article
- Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Frontiers in cell and developmental biology · 2021Article
- Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa.Frontiers in cell and developmental biology · 2021Article
- ProAKAP4 as Novel Molecular Marker of Sperm Quality in Ram: An Integrative Study in Fresh, Cooled and Cryopreserved Sperm.Biomolecules · 2020Article
- Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality.International journal of molecular sciences · 2020Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mammalian sperm must undergo a set of structural and functional changes collectively termed as capacitation to ensure a successful oocyte fertilization. However, capacitation can be compromised by cryopreservation procedures, which alter the proteome and longevity of sperm. To date, how the protein changes induced by cryopreservation could affect the acquisition of sperm fertilizing potential remains unexplored. The present study investigated the protein profile of ram sperm during in vitro capacitation before and after cryopreservation to elucidate the impact of cryopreservation on sperm capacitation at a molecular level. Fresh and cryopreserved ram sperm were incubated under capacitating (CAP) and non-capacitating (NC) conditions for 240 min. The sperm proteome of these four treatments was analyzed and compared at different incubation times using reverse phase liquid chromatography coupled to mass spectrometry (RP-LC-MS/MS). The comparison between fresh and cryopreserved sperm suggested that cryopreservation facilitated an apoptosis-stress response and redox process, while the comparison between sperm incubated in CAP and NC conditions showed that capacitation increased those biological processes associated with signaling, metabolism, motility, and reproductive processes. In addition, 14 proteins related to mitochondrial activity, sperm motility, oocyte recognition, signaling, spermatogenesis, and the apoptosis-stress response underwent significant changes in abundance over time when fresh and cryopreserved sperm incubated in CAP and NC conditions were compared. Our results indicate that disturbances in a ram sperm proteome after cryopreservation may alter the quality of sperm and its specific machinery to sustain capacitation under in vitro conditions.
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