Evidence map›Paper›PMID 31532916›Full record

ArticleCurrent protocols in neuroscience2019

Fluorescence In Situ Imaging of Dendritic RNAs at Single-Molecule Resolution.

Mona Batish, Sanjay Tyagi

Open access · greenAbstract read
In one paragraph

Article in Current protocols in neuroscience, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
0.5field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 17 citations in OpenAlex.

  1. Article
  2. ReducedbioRxiv : the preprint server for biology · 2026
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  15. Stress granule formation, disassembly, and composition are regulated by alphavirus ADP-ribosylhydrolase activity.Proceedings of the National Academy of Sciences of the United States of America · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Mona BatishDepartment of Medical and Molecular Sciences and Department of Biological Sciences, University of Delaware, Newark, Delaware.
Sanjay TyagiPublic Health Research Institute, New Jersey Medical School, Rutgers University, Newark, New Jersey.
Rutgers, The State University of New Jersey · USUniversity of Delaware · US

Funding

Background free amplified single-molecule FISH for in situ and flow cytometric applicationsR01CA227291 · NCI · RBHS-NEW JERSEY MEDICAL SCHOOL · PI TYAGI, SANJAY · 2018 to 2022
$2.8M
DETECTION OF CHROMOSOMAL TRANSLOCATIONS BY SINGLE MOLECULE RNA IMAGINGDP5OD012160 · OD · UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL · PI BATISH, MONA · 2012 to 2016
$2.1M
NCI NIH HHS R01 CA227291NIH HHS DP5 OD012160
6 · The paper itself

Abstract

RNA localization is an important step in gene regulation. Imaging RNAs in fixed and live cells provides contextual information about RNA distribution in the cells. Here, we provide detailed protocols for performing single-molecule fluorescence in situ hybridization (smFISH). smFISH detects mRNA molecules at single-molecule resolution in fixed neuronal cells using ∼50 small oligonucleotide probes for each mRNA. The technique has been successfully applied to understand RNA localization and distribution in various biological systems, ranging from Drosophila to the mammalian nervous system. The probes are small enough to bind to structured RNAs or RNAs that are part of RNA-protein complexes, thereby accounting for ∼85% of the total RNA; this enables a level of sensitivity equivalent to that of quantitative real-time PCR, but with anatomical resolution. © 2019 by John Wiley & Sons, Inc.

Indexed as

In Situ Hybridization, FluorescenceNanotechnologyAnimalsDendritesFluorescent DyesOligonucleotide ProbesRNARNA, MessengerFluorescent DyesOligonucleotide ProbesRNARNA, Messengerdendritic RNAfluorescence in situ hybridization (FISH)RNA imagingRNA localizationsingle molecule

Identifiers

PMID31532916
PMCPMC6752217
OpenAlexW2961799510

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.