Evidence map›Paper›PMID 31531958›Full record

ArticleObesity (Silver Spring, Md.)2019

Macrophage-Derived microRNA-155 Increases in Obesity and Influences Adipocyte Metabolism by Targeting Peroxisome Proliferator-Activated Receptor Gamma.

Jeanie B Tryggestad, April M Teague, David P Sparling, Shaoning Jiang, Steven D Chernausek

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Obesity (Silver Spring, Md.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed, 1 pooled it
1.3field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 1 synthesis or guideline pooled it, 64 citations in OpenAlex.

  1. PPAR-γ Gene Expression in Human Adipose Tissue Is Associated with Weight Loss After Sleeve Gastrectomy.Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract · 2022
    Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Jeanie B TryggestadUniversity of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA.ORCID 0000-0002-5050-0850
April M TeagueUniversity of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA.
David P SparlingUniversity of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA.ORCID 0000-0001-5940-1544
Shaoning JiangUniversity of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA.
Steven D ChernausekUniversity of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA.
University of Oklahoma Health Sciences Center · US

Funding

In utero exposure to diabetes and future cardiometabolic risk: the role of miRNAK23DK106533 · NIDDK · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI TRYGGESTAD, JEANIE BEATRICE · 2016 to 2021
$638k
The role of adipocyte gamma-secretase in regulation of inflammatory signalingK08DK115797 · NIDDK · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI SPARLING, DAVID · 2017 to 2019
$376k
NIDDK NIH HHS K08 DK115797NIDDK NIH HHS K23 DK106533NIDDK NIH HHS L30 DK114995
6 · The paper itself

Abstract

objectiveThis study aimed to investigate cellular sources of microRNAs (miRNA) within adipose tissue and the impact of obesity on miRNA expression, as well as to examine targets of miRNAs.

methodsmiRNA expression by quantitative polymerase chain reaction was examined in adipocytes, adipose tissue macrophages (ATM), and peripheral blood mononuclear cells from and individuals with normal weight and with obesity. Differentiated 3T3-L1 adipocytes were cocultured with macrophages, and 3T3-L1 and differentiated human mesenchymal stem cells were transfected with miR-155, with peroxisome proliferator-activated receptor gamma (PPAR-γ) and solute carrier family 2 member 4 (GLUT4) abundance measured via Western blot analysis.

resultsAbundance of miR-155 and miR-210 was increased in ATM of participants with obesity by 6.7-fold and 2.9-fold (P = 0.002 and P = 0.013, respectively). miR-130b expression was increased 1.8-fold in ATM and 4.3-fold in adipocytes from participants with obesity (P = 0.007 and P = 0.02, respectively). PPARG mRNA expression decreased 32% (P = 0.044) in adipocytes from individuals with obesity. In 3T3-L1 cells exposed to macrophages, PPARG expression decreased 99.4% (P = 0.02). PPAR-γ protein content declined 75% (P = 0.001) in 3T3-L1 cells transfected with miR-155. GLUT4 protein levels were reduced by 55% (P = 0.021) in differentiated human mesenchymal stem cells exposed to miR-155.

conclusionsAdipose tissue miRNAs are influenced in a cell type-specific fashion by obesity, with macrophage miR-155 potentially impacting neighboring adipocytes.

Indexed as

3T3-L1 CellsAdipocytesAdolescentAdultAnimalsCells, CulturedFemaleGene Expression RegulationHumansLipid MetabolismMacrophagesMaleMiceMicroRNAsObesityPPAR gammaMicroRNAsMIRN155 microRNA, humanPPAR gammaPPARG protein, human

Identifiers

PMID31531958
PMCPMC6832842
OpenAlexW2974261710

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.