ArticleBiomicrofluidics2019
An electrochemiluminescent sensing matrix for real-time probing of cell-output reactive oxygen species.
Article in Biomicrofluidics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 authors at 1 institution in 1 country.
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Abstract
Herein, a novel cell-based electrochemiluminescent (ECL) sensing matrix was developed for probing reactive oxygen species (ROSs) produced from mouse macrophage cells. Uniformly sized Au nanoparticles (AuNPs) with an average diameter of 16 nm were decorated on the surface of indium tin oxide (ITO) glass through the connection of hydrolyzed 3-aminopropyl trimethoxysilane (APTMS) serving as a sensor substrate. Then, the surface was covered with a poly-l-lysine thin film, where mouse macrophage cells were successfully cultured. The morphology of the electrodes obtained was characterized by scanning electron microscopy and atomic force microscopy, and their electrochemical properties were investigated by electrochemical impedance spectroscopy. A linear response was observed from the AuNPs/APTMS/ITO substrate with a sensitivity of 0.465 units per mg/l of H
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