ArticleCentral-European journal of immunology2019
MiR-214 regulates CD3ζ expression in T cells.
Article in Central-European journal of immunology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionT-cell activation requires the T-cell receptor (TCR)-CD3 complex, which integrates and transduces signals. CD3ζ plays a vital role in TCR signalling by mediating T-cell activation. Abnormal CD3ζ expression is a common characteristic of haematological malignancies with T-cell immune dysfunction or autoimmune diseases. Targeted regulation of CD3ζ expression by either direct or indirect approaches is important for regulating T-cell activation. AIM OF THE STUDY: In this study, we focused on identifying miRNAs that may regulate CD3ζ expression. MATERIAL AND
methodsThree microRNA target search algorithms (TargetScan, PicTar, and microrna.org) were used to identify hypothetical miRNAs that target CD3ζ in T cells. Of the predicted miRNAs, miR-214 was chosen and validated to determine whether miR-214 directly binds to the CD3ζ 3'-UTR and regulates CD3ζ expression by luciferase reporter assays, real-time PCR, and Western blotting.
resultsThe results indicate that miR-214 specifically binds the CD3ζ 3'-UTR, and miR-214 mimics remarkably reduce the expression of CD3ζ in MOLT-4 cells.
conclusionsWe identify for the first time that miR-214 targets expression in MOLT-4 cells, suggesting that miR-214 might negatively regulate T-cell activation by targeting CD3ζ.
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