ArticleScientific reports2019
Within-host evolution of bovine Staphylococcus aureus selects for a SigB-deficient pathotype characterized by reduced virulence but enhanced proteolytic activity and biofilm formation.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- Insertion sequence elements associated with Staphylococcus epidermidis evolution in persistent orthopaedic device-related infections.BMC genomics · 2026Article
- Evaluating the performance of Fourier transform infrared spectroscopy for typing and outbreak investigation of methicillin-resistant and -susceptibleMicrobiology spectrum · 2026Article
- Bacterial extracellular vesicles as recyclable nutrient reservoirs.Nature communications · 2026Article
- Time-associated phenotypic changes related to virulence and immune interaction of Staphylococcus aureus during relapsing infection within individual hosts.BMC infectious diseases · 2026Article
- Staphylococcus aureus's golden-yellow pigment staphyloxanthin: production enhancement, analytical characterization, and biological attributes.Microbial cell factories · 2026Review
- Resisting the resistance: the antimicrobial peptide DGL13K selects for small colony variants ofJournal of bacteriology · 2025Article
- Genome-wide analysis of fitness determinants of Staphylococcus aureus during growth in milk.PLoS pathogens · 2025Article
- A Global Comparative Genomic Analysis of Major Bacterial Pathogens in Bovine Mastitis and Lameness.Animals : an open access journal from MDPI · 2025Review
- Genomic characterization ofFrontiers in microbiology · 2025Article
- Immunoproteomic analysis of the serum IgG response to cell wall-associated proteins of Staphylococcus aureus strains belonging to CC97 and CC151.Veterinary research · 2023Article
- Bacterial-fungal interactions promote parallel evolution of global transcriptional regulators in a widespread Staphylococcus species.The ISME journal · 2023Article
- Prevalence of the SigB-Deficient Phenotype among ClinicalAntibiotics (Basel, Switzerland) · 2023Article
- Usefulness of molecular typing methods for epidemiological and evolutionary studies ofSaudi journal of biological sciences · 2022Review
- Insight of Melatonin: The Potential of Melatonin to Treat Bacteria-Induced Mastitis.Antioxidants (Basel, Switzerland) · 2022Review
- Article
- Progress towards the Elusive Mastitis Vaccines.Vaccines · 2022Review
- Article
- Within-Host Adaptation ofInternational journal of molecular sciences · 2021Article
- Staphylococcus aureus in Agriculture: Lessons in Evolution from a Multispecies Pathogen.Clinical microbiology reviews · 2021Review
- Biofilm Research in Bovine Mastitis.Frontiers in veterinary science · 2021Review
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
Abstract
Staphylococcus aureus is a major cause of bovine mastitis, commonly leading to long-lasting, persistent and recurrent infections. Thereby, S. aureus constantly refines and permanently adapts to the bovine udder environment. In this work, we followed S. aureus within-host adaptation over the course of three months in a naturally infected dairy cattle with chronic, subclinical mastitis. Whole genome sequence analysis revealed a complete replacement of the initial predominant variant by another isogenic variant. We report for the first time within-host evolution towards a sigma factor SigB-deficient pathotype in S. aureus bovine mastitis, associated with a single nucleotide polymorphism in rsbU (G368A → G122D), a contributor to SigB-functionality. The emerged SigB-deficient pathotype exhibits a substantial shift to new phenotypic traits comprising strong proteolytic activity and poly-N-acetylglucosamine (PNAG)-based biofilm production. This possibly unlocks new nutritional resources and promotes immune evasion, presumably facilitating extracellular persistence within the host. Moreover, we observed an adaptation towards attenuated virulence using a mouse infection model. This study extends the role of sigma factor SigB in S. aureus pathogenesis, so far described to be required for intracellular persistence during chronic infections. Our findings suggest that S. aureus SigB-deficiency is an alternative mechanism for persistence and underpin the clinical relevance of staphylococcal SigB-deficient variants which are consistently isolated during human chronic infections.
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