Trial reportBJOG : an international journal of obstetrics and gynaecology2020
Association of the eukaryotic vaginal virome with prophylactic antibiotic exposure and reproductive outcomes in a subfertile population undergoing in vitro fertilisation: a prospective exploratory study.
Trial report in BJOG : an international journal of obstetrics and gynaecology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 2 syntheses or guidelines pooled it, 29 citations in OpenAlex.
- Viruses in the female lower reproductive tract: a systematic descriptive review of metagenomic investigations.NPJ biofilms and microbiomes · 2024Pooled it
- Antibiotics prior to or at the time of embryo transfer in ART.The Cochrane database of systematic reviews · 2023Pooled it
- Trial
- Review
- Recent Advances in the Role of Bacteriophages in the Aetiology and Therapy of Vaginal Dysbiosis in the Form of Bacterial Vaginosis and the Prevention of Preterm Birth.Microorganisms · 2025Review
- Impact of the Female Genital Microbiota on Outcomes of Assisted Reproductive Techniques.Biomedicines · 2025Review
- The Vaginal Virome in Women's Health and Disease.Microorganisms · 2025Review
- Metagenomic analysis reveals the diversity of the vaginal virome and its association with vaginitis.Frontiers in cellular and infection microbiology · 2025Article
- Effect of preconception antibiotics exposure on female reproductive health and pregnancy outcomes: a systematic review and meta-analysis.EClinicalMedicine · 2024Article
- Correlation between viral infections in male semen and infertility: a literature review.Virology journal · 2024Review
- Altered vaginal eukaryotic virome is associated with different cervical disease status.Virologica Sinica · 2023Article
- Cervicovaginal Human Papillomavirus Genomes, Microbiota Composition and Cytokine Concentrations in South African Adolescents.Viruses · 2023Article
- The endometrial microbiota of women with or without a live birth within 12 months after a first failed IVF/ICSI cycle.Scientific reports · 2023Article
- The Medium Is the Message: Defining a "Normal" Vaginal Microbiome in Healthy Reproductive-Age Women.Reproductive sciences (Thousand Oaks, Calif.) · 2023Article
- Article
- The female reproductive tract virome: understanding the dynamic role of viruses in gynecological health and disease.Current opinion in virology · 2022Review
- ViroMatch: A Computational Pipeline for the Detection of Viral Sequences from Complex Metagenomic Data.Microbiology resource announcements · 2021Article
- Controlled ovarian stimulation and progesterone supplementation affect vaginal and endometrial microbiota in IVF cycles: a pilot study.Journal of assisted reproduction and genetics · 2020Article
- The Vaginal Virome-Balancing Female Genital Tract Bacteriome, Mucosal Immunity, and Sexual and Reproductive Health Outcomes?Viruses · 2020Review
- Diversity of Vaginal Microbiome in Pregnancy: Deciphering the Obscurity.Frontiers in public health · 2020Review
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveThe objective of this study was to use high-throughput sequencing to describe the vaginal eukaryotic DNA virome in patients undergoing in vitro fertilisation (IVF) to examine associations between the vaginal virome, antibiotic exposure and IVF outcomes.
designProspective exploratory study.
settingSingle academic fertility centre. POPULATION: Subfertile women age 18-43 years undergoing their first IVF cycle with a fresh embryo transfer.
methodsThe primary exposure was prophylactic azithromycin or no azithromycin before IVF. A mid-vaginal swab was obtained at the time of embryo transfer for virome analysis.
main outcome measuresThe primary outcomes compared between exposure groups were characteristics of vaginal virome and clinical pregnancy rates. Secondary outcomes were virome associations with number of oocytes retrieved, number of blastocysts and implantation rate.
resultsTwenty-six women contributed a vaginal swab before embryo transfer. There were no significant differences in IVF outcomes between azithromycin groups. There was no association between viral diversity and clinical pregnancy overall. A higher diversity of herpesviruses and α-papillomaviruses was observed in samples from the azithromycin-treated group compared with the no azithromycin group (P = 0.04). In women that received azithromycin, viral diversity was higher in the group that did not achieve clinical pregnancy compared with those who did (P = 0.06).
conclusionsWe demonstrate that the vaginal eukaryotic virome in women undergoing IVF is associated with antibiotic exposure. Additionally, we demonstrate an inverse trend between viral diversity and pregnancy, with a higher number of viruses detected associated with failure to achieve clinical pregnancy in the azithromycin group. TWEETABLE ABSTRACT: Higher viral diversity is associated with prophylactic antibiotic exposure in subfertile women undergoing IVF.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.