ArticleChembiochem : a European journal of chemical biology2019
Optimization of the Inverted Emulsion Method for High-Yield Production of Biomimetic Giant Unilamellar Vesicles.
Article in Chembiochem : a European journal of chemical biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed.
- Environmental Impact of Synthetic Cell Technology: Review of Life Cycle Assessment Data for Feedstocks and Production Methods.Chembiochem : a European journal of chemical biology · 2026Review
- QuantGUV: Quantifying Encapsulation Efficiency of Small Molecules in Giant Unilamellar Vesicles.ACS applied materials & interfaces · 2026Article
- Employing molecular beacons to assess in vitro transcription with single-molecule resolution.Scientific reports · 2026Article
- A robust method for on-chip production and manipulation of lipid vesicles by inverted emulsion.Cell reports methods · 2026Article
- Engineering synthetic cells with intramembrane domains possessing distinct bilayer asymmetries.Nature communications · 2026Article
- Light-induced assembly and repeatable actuation in CaNature communications · 2026Article
- Lipid composition effects on the number and size of liposomes formed by the inverted emulsion method.Biophysical journal · 2026Article
- Assembling Lipid Membrane Scaffolds on Microgel-Based Artificial Cells through Vesicle Fusion onto the Hydrogel Network.ACS nano · 2026Article
- Molecular systems engineering of synthetic cells.Nature chemistry · 2026Review
- Design of a Trigger-Responsive Photothermal Vesicle-Based Cargo Delivery Platform.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Nuclear Assembly in Giant Unilamellar Vesicles Encapsulating Xenopus Egg Extract.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- On-the-Fly Microfluidic Control of Giant Vesicle Compositions Validated by DNA Surface Charge Sensors.ACS nano · 2025Article
- Liposome-EncapsulatedACS synthetic biology · 2025Article
- Remodeling of lipid-foam prototissues by network-wide tension fluctuations induced by active particles.Nature communications · 2025Article
- A New Means to Generate Liposomes by Rehydrating Engineered Lipid Nanoconstructs.Micromachines · 2025Article
- Challenges in observing transcription-translation for bottom-up synthetic biology.QRB discovery · 2025Review
- Imidazole Headgroup Phospholipid Shows Asymmetric Distribution in Vesicles and Zinc-Dependent Esterase Activity.Biomolecules · 2024Article
- Several common methods of making vesicles (except an emulsion method) capture intended lipid ratios.Biophysical journal · 2024Article
- Curvature-Assisted Vesicle Explosion Under Light-Induced Asymmetric Oxidation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Effect of leaflet asymmetry on the stretching elasticity of lipid bilayers with phosphatidic acid.Biophysical journal · 2024Article
1 more citing papers are in PubMed but not listed here.
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4 authors.
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Abstract
In the field of bottom-up synthetic biology, lipid vesicles provide an important role in the construction of artificial cells. Giant unilamellar vesicles (GUVs), due to their membrane's similarity to natural biomembranes, have been widely used as cellular mimics. So far, several methods exist for the production of GUVs with the possibility to encapsulate biological macromolecules. The inverted emulsion-based method is one such technique, which has great potential for rapid production of GUVs with high encapsulation efficiencies for large biomolecules. However, the lack of understanding of various parameters that affect production yields has resulted in sparse adaptation within the membrane and bottom-up synthetic biology research communities. Here, we optimize various parameters of the inverted emulsion-based method to maximize the production of GUVs. We demonstrate that the density difference between the emulsion droplets, oil phase, and the outer aqueous phase plays a crucial role in vesicle formation. We also investigated the impact that centrifugation speed/time, lipid concentration, pH, temperature, and emulsion droplet volume has on vesicle yield and size. Compared to conventional electroformation, our preparation method was not found to significantly alter the membrane mechanical properties. Finally, we optimize the parameters to minimize the time from workbench to microscope and in this way open up the possibility of time-sensitive experiments. In conclusion, our findings will promote the usage of the inverted emulsion method for basic membrane biophysics studies as well as the development of GUVs for use as future artificial cells.
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