ArticleRegenerative therapy2019
In vitro periodontal ligament cell expansion by co-culture method and formation of multi-layered periodontal ligament-derived cell sheets.
Article in Regenerative therapy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
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- Assessing bone regeneration potential of 3D scaffold-free cell pellets from periodontal ligament and bone marrow stem cells.BMC biotechnology · 2025Article
- Phytochemical Profile and Anticancer Activity of Achillea conferta Leaf Extracts: Insights Into Antioxidant Properties.Chemistry & biodiversity · 2025Article
- Impact of mechanotransduction on gene expression changes in periodontal ligament during orthodontic tooth movement.Journal of bone and mineral metabolism · 2025Article
- Evaluation of Random and Aligned Polycaprolactone Nanofibrous Electrospun Scaffold for Human Periodontal Ligament Engineering in Biohybrid Titanium Implants.International journal of dentistry · 2024Article
- Assessing the cytotoxicity of phenolic and terpene fractions extracted from IraqiF1000Research · 2023Article
- Bio-hybrid dental implants prepared using stem cells with β-TCP-coated titanium and zirconia.Journal of periodontal & implant science · 2022Article
- Influence of Fiber Laser (1064 nm) on Shear Bond Strength of Titanium Abutment and Resin Cement.Journal of lasers in medical sciences · 2022Article
- Biomaterials and biotechnology for periodontal tissue regeneration: Recent advances and perspectives.Journal of dental research, dental clinics, dental prospects · 2022Review
- Periodontal ligament stem cell-based bioactive constructs for bone tissue engineering.Frontiers in bioengineering and biotechnology · 2022Review
- Effects of Long Durations of RF-Magnetron Sputtering Deposition of Hydroxyapatite on Titanium Dental Implants.European journal of dentistry · 2021Article
- Effect ofExperimental and therapeutic medicine · 2021Article
- Preparing polycaprolactone scaffolds using electrospinning technique for construction of artificial periodontal ligament tissue.Journal of Taibah University Medical Sciences · 2020Article
- Enhanced VEGF/VEGF-R and RUNX2 Expression in Human Periodontal Ligament Stem Cells Cultured on Sandblasted/Etched Titanium Disk.Frontiers in cell and developmental biology · 2020Article
- Article
- Bone regeneration capacities of alveolar bone mesenchymal stem cells sheet in rabbit calvarial bone defect.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivePer-implantitis is one of the implant treatment complications. Dentists have failed to restore damaged periodontium by using conventional therapies. Tissue engineering (stem cells, scaffold and growth factors) aims to reconstruct natural tissues. The paper aimed to isolate both periodontal ligament stem cells (PDLSCs) and bone marrow mesenchymal stem cells (BMMSCs) and use them in a co-culture method to create three-layered cell sheets for reconstructing natural periodontal ligament (PDL) tissue. MATERIALS AND
methodsBMMSCs were isolated from rabbit tibia and femur, and PDLSC culture was established from the lower right incisor. The cells were co-cultured to induce BMMSC differentiation into PDL cells. Cell morphology, stem cells and PDL-specific markers (CD90, CD34, and periostin) were also detected using immunofluorescent assay. Co-cultured cell monolayers were detached using temperature-responsive tissue culture dishes and collagen graft to create the three-layer construct. The 3D-engineered tissue was examined histologically and by field emission scanning electron microscopy (FESEM).
resultsBMMSCs co-cultured with PDLSCs successfully induced more PDL cells. The newly induced PDL cells exhibited periostin and CD90 expression. Fluorescence green intensity was measured for the co-cultured cells that were stained with periostin, the mean fluorescence green intensity (periostin expression) was significantly higher for the newly induced PDL cells after 1, 2, and 3 weeks when compared with control (BM-MSCs), at 21 days non-significant difference was measured when compared with control (PDLSCs).The results showed the successful formation of 3D multilayer PDL tissue. Histological cross-section showed cell sheets and the stable adhesion between them. FESEM examination was conducted for the cross-section, showing three-layered cell sheets with stable adhesion between cells.
conclusionsThe results of this paper report that the three layered-cell sheets were successfully constructed by the novel use of collagen graft as a scaffold to be used in treatment of periodontitis and to envelop the dental implants to create biohybrid implant.
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