Evidence map›Paper›PMID 31527759›Full record

ArticleScientific reports2019

Nuclease dead Cas9 is a programmable roadblock for DNA replication.

Kelsey S Whinn, Gurleen Kaur, Jacob S Lewis, Grant D Schauer, Stefan H Mueller, Slobodan Jergic, Hamish Maynard, Zhong Yan Gan, Matharishwan Naganbabu, Marcel P Bruchez and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.

0numbers the graph read from it
0cells of the map it votes in
42citing papers in PubMed
2.3field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

42 citing papers in PubMed, 62 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Review
  6. Article
  7. Review
  8. Article
  9. DNA Polymerase Locks Replication Fork Under Stress.bioRxiv : the preprint server for biology · 2024
    Article
  10. Review
  11. Article
  12. Review
  13. Review
  14. Article
  15. Review
  16. Modeling specific aneuploidies: from karyotype manipulations to biological insights.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2023
    Review
  17. Article
  18. Deletions of DNA in cancer and their possible uses for therapy.BioEssays : news and reviews in molecular, cellular and developmental biology · 2023
    Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 2 countries.

Kelsey S WhinnSchool of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, New South Wales, 2522, Australia.ORCID http://orcid.org/0000-0003-4032-0212
Gurleen KaurSchool of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, New South Wales, 2522, Australia.ORCID http://orcid.org/0000-0003-4466-2279
Jacob S LewisSchool of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, New South Wales, 2522, Australia.ORCID http://orcid.org/0000-0002-9945-6133
Grant D SchauerHoward Hughes Medical Institute, Rockefeller University, New York, NY, 10065, USA.ORCID http://orcid.org/0000-0002-6200-1311
Stefan H MuellerSchool of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, New South Wales, 2522, Australia.ORCID http://orcid.org/0000-0002-0930-2000
Slobodan JergicSchool of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, New South Wales, 2522, Australia.
Hamish MaynardSchool of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, New South Wales, 2522, Australia.
Zhong Yan GanSchool of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, New South Wales, 2522, Australia.ORCID http://orcid.org/0000-0001-5755-7780
Matharishwan NaganbabuDepartment of Chemistry and Molecular Biosensors and Imaging Center, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania, 15213, USA.ORCID http://orcid.org/0000-0001-5121-1412
Marcel P BruchezDepartment of Chemistry and Molecular Biosensors and Imaging Center, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania, 15213, USA.ORCID http://orcid.org/0000-0002-7370-4848
Michael E O'DonnellHoward Hughes Medical Institute, Rockefeller University, New York, NY, 10065, USA.ORCID http://orcid.org/0000-0001-9002-4214
Nicholas E DixonSchool of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, New South Wales, 2522, Australia.ORCID http://orcid.org/0000-0002-5958-6945
Antoine M van OijenSchool of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, New South Wales, 2522, Australia. vanoijen@uow.edu.au.ORCID http://orcid.org/0000-0002-1794-5161
Harshad GhodkeSchool of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, New South Wales, 2522, Australia. harshad@uow.edu.au.ORCID http://orcid.org/0000-0002-6628-876X
University of Wollongong · AUHoward Hughes Medical Institute · USImaging Center · US

Funding

Mechanisms of Cell Regulation and TransformationT32CA009673 · NCI · ROCKEFELLER UNIVERSITY · PI KAPOOR, TARUN M. · 1991 to 2018
$7.0M
Biochemical Mechanism and Structure of the Eukaryotic Replication ForkR01GM115809 · NIGMS · ROCKEFELLER UNIVERSITY · PI O'DONNELL, MICHAEL E · 2015 to 2022
$2.6M
Targeted fluorescent indicators for endothelial physiology: Ca(II), ROS, NOR01EB017268 · NIBIB · CARNEGIE-MELLON UNIVERSITY · PI BRUCHEZ, MARCEL P, ST CROIX, CLAUDETTE MARIE · 2013 to 2016
$2.5M
Fluorogen Activating Peptide-Based FRET to Quantify FceRI Activation MechanismsR01GM114075 · NIGMS · CARNEGIE-MELLON UNIVERSITY · PI BRUCHEZ, MARCEL P, LIDKE, DIANE · 2015 to 2018
$1.2M
BYPASS MECHANISMS IN EUKARYOTIC REPLICATIONR00GM126143 · NIGMS · COLORADO STATE UNIVERSITY · PI SCHAUER, GRANT · 2019 to 2021
$747k
Bypass Mechanisms in Eukaryotic ReplicationK99GM126143 · NIGMS · ROCKEFELLER UNIVERSITY · PI SCHAUER, GRANT · 2018 to 2018
$90k
NCI NIH HHS T32 CA009673NIBIB NIH HHS R01 EB017268NIGMS NIH HHS K99 GM126143NIGMS NIH HHS R00 GM126143NIGMS NIH HHS R01 GM114075NIGMS NIH HHS R01 GM115809
6 · The paper itself

Abstract

Limited experimental tools are available to study the consequences of collisions between DNA-bound molecular machines. Here, we repurpose a catalytically inactivated Cas9 (dCas9) construct as a generic, novel, targetable protein-DNA roadblock for studying mechanisms underlying enzymatic activities on DNA substrates in vitro. We illustrate the broad utility of this tool by demonstrating replication fork arrest by the specifically bound dCas9-guideRNA complex to arrest viral, bacterial and eukaryotic replication forks in vitro.

Indexed as

CRISPR-Associated Protein 9CRISPR-Cas SystemsDNA, BacterialDNA ReplicationEscherichia coliRNA, Guide, CRISPR-Cas SystemsStreptococcus pyogenesCRISPR-Associated Protein 9DNA, BacterialRNA, Guide, CRISPR-Cas Systems

Identifiers

PMID31527759
PMCPMC6746809
OpenAlexW2973731348

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.