ArticleScientific reports2019
Benchmarking urine storage and collection conditions for evaluating the female urinary microbiome.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
33 citing papers in PubMed, 46 citations in OpenAlex.
- Longitudinal Impact of Methenamine Hippurate on the Urobiome of Postmenopausal Women With Recurrent UTIs.International urogynecology journal · 2026Article
- Diagnostic Accuracy of Urine Microbial Diversity and Urine Neutrophil Gelatinase-Associated Lipocalin for the Diagnosis of Urinary Tract Infections in Children with Spina Bifida.The Journal of pediatrics · 2026Article
- Basic Microbiome Analysis: Analytical Steps from Sampling to Sequencing.Microorganisms · 2026Review
- The Urinary Microbiota and the Gut-Bladder Axis in Bladder Cancer.International journal of molecular sciences · 2025Review
- Gynecologic Oncology Patients have Urobiomes that are Similar to Women Without Gynecologic Cancer.International urogynecology journal · 2025Article
- Urinary Metabolomic Profile is Minimally Impacted by Common Storage Conditions and Additives.International urogynecology journal · 2025Article
- Exploring urinary microbiome: insights into neurogenic bladder and improving management of urinary tract infections.Frontiers in cellular and infection microbiology · 2025Review
- Characterizing the urobiome in geriatric males with chronic indwelling urinary catheters: an exploratory longitudinal study.Microbiology spectrum · 2024Article
- Metagenome-based microbial community analysis of urine-derived fertilizer.BMC microbiology · 2024Article
- The Role of the Urobiome in Kidney Transplantation: A Systematic Review.Transplantation direct · 2024Article
- Current Trends and Challenges of Microbiome Research in Bladder Cancer.Current oncology reports · 2024Review
- The Urinary Microbiome in Health and Disease: Relevance for Bladder Cancer.International journal of molecular sciences · 2024Review
- Effects of variation in sample storage conditions and swab order on 16S vaginal microbiome analyses.Microbiology spectrum · 2024Article
- The RISE FOR HEALTH study: Methods for in-person assessment and biospecimen collection.Neurourology and urodynamics · 2023Article
- Effects of Variation in Urine Sample Storage Conditions on 16S Urogenital Microbiome Analyses.mSystems · 2023Article
- Microbiome-Based Stain Analyses in Crime Scenes.Applied and environmental microbiology · 2023Review
- Urine microbiome in individuals with an impaired immune system.Frontiers in cellular and infection microbiology · 2023Review
- Review
- The pediatric urobiome in genitourinary conditions: a narrative review.Pediatric nephrology (Berlin, Germany) · 2022Review
- Choice of 16S Ribosomal RNA Primers Impacts Male Urinary Microbiota Profiling.Frontiers in cellular and infection microbiology · 2022Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Standardized conditions for collection, preservation and storage of urine for microbiome research have not been established. We aimed to identify the effects of the use of preservative AssayAssure® (AA), and the effects of storage time and temperatures on reproducibility of urine microbiome results. We sequenced the V3-4 segment of the 16S rRNA gene to characterize the bacterial community in the urine of a cohort of women. Each woman provided a single voided urine sample, which was divided into aliquots and stored with and without AA, at three different temperatures (room temperature [RT], 4 °C, or -20 °C), and for various time periods up to 4 days. There were significant microbiome differences in urine specimens stored with and without AA at all temperatures, but the most significant differences were observed in alpha diversity (estimated number of taxa) at RT. Specimens preserved at 4 °C and -20 °C for up to 4 days with or without AA had no significant alpha diversity differences. However, significant alpha diversity differences were observed in samples stored without AA at RT. Generally, there was greater microbiome preservation with AA than without AA at all time points and temperatures, although not all results were statistically significant. Addition of AA preservative, shorter storage times, and colder temperatures are most favorable for urinary microbiome reproducibility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.