ArticleScientific reports2019
All-optical Reflection-mode Microscopic Histology of Unstained Human Tissues.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Photoacoustic imaging for cutaneous melanoma assessment: a comprehensive review.Journal of biomedical optics · 2024Pooled it
- Deep UV laser scanning microscopy with integrated 1050 nm spectral-domain optical coherence tomography for multi-contrast tissue imaging.Biomedical optics express · 2025Article
- Review of Non-Invasive Imaging Technologies for Cutaneous Melanoma.Biosensors · 2025Review
- Metabolic light absorption, scattering, and emission (MetaLASE) microscopy.Science advances · 2024Article
- Deep learning-enabled realistic virtual histology with ultraviolet photoacoustic remote sensing microscopy.Nature communications · 2023Article
- Niche preclinical and clinical applications of photoacoustic imaging with endogenous contrast.Photoacoustics · 2023Review
- Sensing Cells-Peptide Hydrogel Interaction In Situ via Scanning Ion Conductance Microscopy.Cells · 2022Article
- Deformable mirror-based photoacoustic remote sensing (PARS) microscopy for depth scanning.Biomedical optics express · 2022Article
- Virtual histological staining of label-free total absorption photoacoustic remote sensing (TA-PARS).Scientific reports · 2022Article
- Ultraviolet photoacoustic microscopy with tissue clearing for high-contrast histological imaging.Photoacoustics · 2022Article
- Multimodal 3D photoacoustic remote sensing and confocal fluorescence microscopy imaging.Journal of biomedical optics · 2021Article
- Three-dimensional virtual histology in unprocessed resected tissues with photoacoustic remote sensing (PARS) microscopy and optical coherence tomography (OCT).Scientific reports · 2021Article
- Live feedback and 3D photoacoustic remote sensing.Quantitative imaging in medicine and surgery · 2021Article
- Towards virtual biopsies of gastrointestinal tissues using photoacoustic remote sensing microscopy.Quantitative imaging in medicine and surgery · 2021Article
- Photoacoustic Neuroimaging - Perspectives on a Maturing Imaging Technique and its Applications in Neuroscience.Frontiers in neuroscience · 2021Review
- Histopathology for Mohs micrographic surgery with photoacoustic remote sensing microscopy.Biomedical optics express · 2021Article
- Towards non-contact photoacoustic imaging [review].Photoacoustics · 2020Review
- Reflection-mode virtual histology using photoacoustic remote sensing microscopy.Scientific reports · 2020Article
- Improving maximal safe brain tumor resection with photoacoustic remote sensing microscopy.Scientific reports · 2020Article
- Rapid High-Resolution Mosaic Acquisition for Photoacoustic Remote Sensing.Sensors (Basel, Switzerland) · 2020Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Surgical oncologists depend heavily on visual field acuity during cancer resection surgeries for in-situ margin assessment. Clinicians must wait up to two weeks for results from a pathology lab to confirm a post-operative diagnosis, potentially resulting in subsequent treatments. Currently, there are no clinical tools that can visualize diagnostically pertinent tissue information in-situ. Here, we present the first microscopy capable of non-contact label-free visualization of human cellular morphology in a reflection-mode apparatus. This is possible with the recently reported imaging modality called photoacoustic remote sensing microscopy which enables non-contact detection of optical absorption contrast. By taking advantage of the 266-nanometer optical absorption peak of DNA, photoacoustic remote sensing is efficacious in recovering qualitatively similar nuclear information in comparison to that provided by the hematoxylin stain in the gold-standard hematoxylin and eosin (H&E) prepared samples. A photoacoustic remote sensing system was employed utilizing a 266-nanometer pulsed excitation beam to induce photoacoustic pressures within the sample resulting in refractive index modulation of the optical absorber. A 1310-nanometer continuous-wave interrogation beam detects these perturbed regions as back reflected intensity variations due to the changes in the local optical properties. Using this technique, clinically useful histologic images of human tissue samples including breast cancer (invasive ductal carcinoma), tonsil, gastrointestinal, and pancreatic tissue images were formed. These were qualitatively comparable to standard H&E prepared samples.
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