ArticleScientific reports2019
Evaluation and Validation of Reference Genes for Quantitative Real-Time PCR in Helopeltis theivora Waterhouse (Hemiptera: Miridae).
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed.
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- Evaluation of Reference Genes for Quantitative Real-Time PCR Analysis in the Bean Bug,Insects · 2023Article
- Evaluation and validation of reference genes for RT-qPCR gene expression in Naegleria gruberi.Scientific reports · 2023Article
- Evaluation of Reference Genes for Real-Time Quantitative PCR Analysis in Tissues from Bumble Bees (International journal of molecular sciences · 2022Article
- Comparative Transcriptome and Metabolome Analysis of Resistant and SusceptibleFrontiers in plant science · 2022Article
- Selection and validation of reference genes for RT-qPCR-based analyses ofFrontiers in physiology · 2022Article
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- Blood Differential Gene Expression in Patients with Chronic Heart Failure and Systemic Iron Deficiency: Pathways Involved in Pathophysiology and Impact on Clinical Outcomes.Journal of clinical medicine · 2021Article
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- Melatonin reduces inflammation in intestinal cells, organoids and intestinal explants.Inflammopharmacology · 2021Article
- Selection and validation of reference genes for quantitative real-time PCR in the green microalgae Tetraselmis chui.PloS one · 2021Article
- Reference gene selection for qRT-PCR analysis of season- and tissue-specific gene expression profiles in the honey bee Apis mellifera.Scientific reports · 2020Article
- Suitable reference genes for RT-qPCR analysis in Dichelops melacanthus (Hemiptera: Pentatomidae).Molecular biology reports · 2020Article
- Screening and Validation of Reference Genes for RT-qPCR Under Different Honey Bee Viral Infections and dsRNA Treatment.Frontiers in microbiology · 2020Article
- Identification and Evaluation of Reference Genes for Normalization of Gene Expression in Developmental Stages, Sexes, and Tissues of Diaphania caesalis (Lepidoptera, Pyralidae).Journal of insect science (Online) · 2020Article
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7 authors.
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Abstract
Helopeltis theivora Waterhouse is a predominant sucking pest in many tropic economic crops, such as tea, cocoa and coffee. Quantitative real-time PCR (qRT-PCR) is one of the most powerful tools to analyze the gene expression level and investigate the mechanism of insect physiology at transcriptional level. Gene expression studies utilizing qRT-PCR have been applied to numerous insects so far. However, no universal reference genes could be used for H. theivora. To obtain accurate and reliable normalized data in H. theivora, twelve candidate reference genes were examined under different tissues, developmental stages and sexes by using geNorm, NormFinder, BestKeeper, Delta Ct and RefFinder algorithms, respectively. The results revealed that the ideal reference genes differed across the treatments, and the consensus rankings generated from stability values provided by these programs suggested a combination of two genes for normalization. To be specific, RPS3A and Actin were the best suitable reference genes for tissues, RPL13A and GAPDH were suitable for developmental stages, EF1α and RPL13A were suitable for sexes, and RPL13A and RPS3A were suitable for all samples. This study represents the first systematic analysis of reference genes for qRT-PCR experiments in H. theivora, and the results can provide a credible normalization for qRT-PCR data, facilitating transcript profiling studies of functional genes in this insect.
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