Evidence map›Paper›PMID 31527268›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2019

Discordance between eNOS phosphorylation and activation revealed by multispectral imaging and chemogenetic methods.

Emrah Eroglu, Seyed Soheil Saeedi Saravi, Andrea Sorrentino, Benjamin Steinhorn, Thomas Michel

Open access · bronzeAbstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed
4.0field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed, 67 citations in OpenAlex.

  1. Article
  2. Article
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  4. Review
  5. The machinery of healthy vasodilatation: an overview.Pflugers Archiv : European journal of physiology · 2025
    Review
  6. Article
  7. Article
  8. The Ever-Expanding Influence of the Endothelial Nitric Oxide Synthase.Basic & clinical pharmacology & toxicology · 2025
    Review
  9. Article
  10. Article
  11. Article
  12. Article
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  18. Article
  19. Expression of CD70 Modulates Nitric Oxide and Redox Status in Endothelial Cells.Arteriosclerosis, thrombosis, and vascular biology · 2022
    Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Emrah ErogluDivision of Cardiovascular Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115.
Seyed Soheil Saeedi SaraviDivision of Cardiovascular Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115.
Andrea SorrentinoDivision of Cardiovascular Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115.
Benjamin SteinhornDivision of Cardiovascular Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115.
Thomas MichelDivision of Cardiovascular Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115 thomas_michel@hms.harvard.edu.ORCID 0000-0003-1193-9841
Brigham and Women's Hospital · US

Funding

Medical Scientist Training ProgramT32GM007753 · NIGMS · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI WALENSKY, LOREN DAVID · 1985 to 2021
$50.0M
Transgenic CoreP30DK057521 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI KAHN, BARBARA B. · 2000 to 2019
$31.4M
Hydrogen peroxide in endothelial function and dysfunctionR01HL152173 · NHLBI · BRIGHAM AND WOMEN'S HOSPITAL · PI MICHEL, THOMAS · 2021 to 2024
$2.2M
Dynamic tissue-specific modulation of redox stress using chemogeneticsR33HL157918 · NHLBI · BRIGHAM AND WOMEN'S HOSPITAL · PI MICHEL, THOMAS · 2021 to 2022
$1.0M
Chemogenetic approaches to define the roles of redox dysfunction in the cardiomyopathy of agingR21AG063073 · NIA · BRIGHAM AND WOMEN'S HOSPITAL · PI MICHEL, THOMAS · 2019 to 2020
$480k
Austrian Science Fund FWF J 4113NHLBI NIH HHS R01 HL152173NHLBI NIH HHS R33 HL157918NIA NIH HHS R21 AG063073NIDDK NIH HHS P30 DK057521NIGMS NIH HHS T32 GM007753
6 · The paper itself

Abstract

Nitric oxide (NO) synthesized by the endothelial isoform of nitric oxide synthase (eNOS) is a critical determinant of vascular homeostasis. However, the real-time detection of intracellular NO-a free radical gas-has been difficult, and surrogate markers for eNOS activation are widely utilized. eNOS phosphorylation can be easily measured in cells by probing immunoblots with phosphospecific antibodies. Here, we pursued multispectral imaging approaches using biosensors to visualize intracellular NO and Ca

Indexed as

Biosensing TechniquesMolecular ImagingAMP-Activated Protein KinasesCalciumCells, CulturedCytosolEndothelial CellsEnzyme ActivationNitric OxideNitric Oxide SynthaseNitric Oxide Synthase Type IIIPhosphorylationProtein Kinase InhibitorsSignal TransductionAMP-Activated Protein KinasesCalciumNitric OxideNitric Oxide SynthaseNitric Oxide Synthase Type IIIProtein Kinase Inhibitorsendothelial cellsnitric oxide synthasephosphorylationsignal transduction

Identifiers

PMID31527268
PMCPMC6778257
OpenAlexW2973626758

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.