Evidence map›Paper›PMID 31527065›Full record

ArticleBioscience reports2019

Function analysis of differentially expressed microRNAs in TGF-β1-induced cardiac fibroblasts differentiation.

Suxuan Liu, Wen Ke, Yang Liu, Zhenzhen Zhao, Lina An, Xiaohua You, Fan Yang, Xiangqun Yang, Guokun Wang, Xianxian Zhao

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioscience reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. MicroRNAs regulating signaling pathways in cardiac fibrosis: potential role of the exercise training.American journal of physiology. Heart and circulatory physiology · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Suxuan LiuDepartment of Cardiology, Changhai Hospital, Naval Medical University, Shanghai 200433, China.
Wen KeDepartment of Medical Service, 905th Military Hospital, Naval Medical University, Shanghai 200040, China.
Yang LiuDepartment of Cardiovascular Surgery, Institute of Cardiac Surgery, Changhai Hospital, Naval Medical University, Shanghai 200433, China.
Zhenzhen ZhaoAnesthesiology and Intensive Care Medicine, Changhai Hospital, Naval Medical University, Shanghai 200433, China.
Lina AnDepartment of Geriatrics, Shanghai First People's Hospital, Medical College, Shanghai Jiaotong University, Shanghai 200080, China.
Xiaohua YouDepartment of Cardiology, Changhai Hospital, Naval Medical University, Shanghai 200433, China.
Fan YangDepartment of Cardiovascular Surgery, Institute of Cardiac Surgery, Changhai Hospital, Naval Medical University, Shanghai 200433, China.
Xiangqun YangDepartment of Anatomy, Naval Medical University, Shanghai 200433, China.
Guokun WangDepartment of Cardiovascular Surgery, Institute of Cardiac Surgery, Changhai Hospital, Naval Medical University, Shanghai 200433, China.
Xianxian ZhaoDepartment of Cardiology, Changhai Hospital, Naval Medical University, Shanghai 200433, China.
Second Military Medical University · CNShanghai First People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCardiac fibroblasts differentiation plays a critical role in cardiac remodeling and failure, but the underlying molecular mechanisms are still poorly understood. MicroRNAs (miRNAs) had been identified as important regulators during cell differentiation. The aim of the present study was to screen the miRNAs involved in regulation of cardiac fibroblasts differentiation.

methodsThe differentiation of rat cardiac fibroblasts into myofibroblasts was induced by transforming growth factor-β1 (TGF-β1). Small RNA sequencing was then applied to detect the differentially expressed miRNAs.

resultsA total of 450 known miRNAs were detected, and 127 putative novel miRNAs were predicted by miRDeep2 analysis. DEGseq analysis and qRT-PCR confirmed that 24 known miRNAs were differentially expressed in TGF-β1-induced cardiac fibroblasts, including three up-regulated miRNAs and 21 down-regulated miRNAs. After miRNAs target genes prediction by miRanda algorithm, pathway analysis showed that these potential target genes were involved in Calcium signaling pathway, Type II diabetes mellitus, and Glutamatergic synapse pathway, etc. Meanwhile, seven putative miRNAs were also detected differentially expressed during TGF-β1-induced cardiac fibroblasts differentiation.

conclusionsThese differentially expressed miRNAs might play critical roles in cardiac fibroblasts differentiation. Altered expression of miRNAs may yield new insights into the underlying mechanisms of cardiac fibrosis and provide novel mechanism-based therapeutic strategies for cardiac fibrosis.

Indexed as

Gene Expression RegulationAnimalsCalcium SignalingCell DifferentiationFibroblastsFibrosisHeart DiseasesMicroRNAsMyocardiumRatsRats, Sprague-DawleyTransforming Growth Factor beta1MicroRNAsTgfb1 protein, ratTransforming Growth Factor beta1cardiac fibroblastsdeep sequencingdifferentiationmicroRNAstransforming growth factor-β1

Identifiers

PMID31527065
PMCPMC6822545
OpenAlexW2974669710

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.