ArticleJournal of the American Chemical Society2019
Genetically Encoded Photocleavable Linkers for Patterned Protein Release from Biomaterials.
Article in Journal of the American Chemical Society, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed.
- Peptomer Linkers Enable Kinetic Control over Co-Delivery of Multiple Chemotherapeutics.Advanced healthcare materials · 2026Article
- From Light to Life: Molecular Mechanisms and Macroscopic Transformations in Photoresponsive Hydrogels.Polymer science & technology (Washington, D.C.) · 2025Review
- Boolean logic-gated protein presentation through autonomously compiled molecular topology.Nature chemical biology · 2025Article
- Photoinduced chromophore dissociation resulting in aggregation-induced red fluorescence.Communications biology · 2025Article
- PhoCoil: A photodegradable and injectable single-component recombinant protein hydrogel for minimally invasive delivery and degradation.Science advances · 2025Article
- Smart Bio-Nanocoatings with Simple Post-Synthesis Reversible Adjustment.Biomimetics (Basel, Switzerland) · 2025Article
- Light-based fabrication and 4D customization of hydrogel biomaterials.Nature reviews bioengineering · 2025Article
- Three-Color Protein Photolithography with Green, Red, and Far-Red Light.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Bioluminescence Resonance Energy Transfer (BRET)-Mediated Protein Release from Self-Illuminating Photoresponsive Biomaterials.Journal of the American Chemical Society · 2024Article
- Versatile tissue-injectable hydrogels capable of the extended hydrolytic release of bioactive protein therapeutics.Bioengineering & translational medicine · 2024Article
- Improving Photocleavage Efficiency of Photocleavable Protein for Antimicrobial Peptide Histatin 1 Expression.Protein and peptide letters · 2024Article
- Stimuli-Responsive Protein Hydrogels: Their Design, Properties, and Biomedical Applications.Polymers · 2023Review
- Chemical and Biological Engineering Strategies to Make and Modify Next-Generation Hydrogel Biomaterials.Matter · 2023Article
- Multifunctional silk vinyl sulfone-based hydrogel scaffolds for dynamic material-cell interactions.Biomaterials · 2023Article
- 4D Biochemical Photocustomization of Hydrogel Scaffolds for Biomimetic Tissue Engineering.Accounts of materials research · 2023Article
- Engineering Multi-Scale Organization for Biotic and Organic Abiotic Electroactive Systems.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Gigavalent Display of Proteins on Monodisperse Polyacrylamide Hydrogels as a Versatile Modular Platform for Functional Assays and Protein Engineering.ACS central science · 2022Article
- The Art of Engineering Biomimetic Cellular Microenvironments.ACS biomaterials science & engineering · 2021Article
- Click-functionalized hydrogel design for mechanobiology investigations.Molecular systems design & engineering · 2021Article
- Multiphoton Laser Fabrication of Hybrid Photo-Activable Biomaterials.Sensors (Basel, Switzerland) · 2021Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Given the critical role that proteins play in almost all biological processes, there is great interest in controlling their presentation within and release from biomaterials. Despite such outstanding enthusiasm, previously developed strategies in this regard result in ill-defined and heterogeneous populations with substantially decreased activity, precluding their successful application to fragile species including growth factors. Here, we introduce a modular and scalable method for creating monodisperse, genetically encoded chimeras that enable bioactive proteins to be immobilized within and subsequently photoreleased from polymeric hydrogels. Building upon recent developments in chemoenzymatic reactions, bioorthogonal chemistry, and optogenetics, we tether fluorescent proteins, model enzymes, and growth factors site-specifically to gel biomaterials through a photocleavable protein (PhoCl) that undergoes irreversible backbone photoscission upon exposure to cytocompatible visible light (λ ≈ 400 nm) in a dose-dependent manner. Mask-based and laser-scanning lithographic strategies using commonly available light sources are employed to spatiotemporally pattern protein release from hydrogels while retaining their full activity. The photopatterned epidermal growth factor presentation is exploited to promote anisotropic cellular proliferation in 3D. We expect these methods to be broadly useful for applications in diagnostics, drug delivery, and regenerative medicine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.