ArticleAnalytical chemistry2019
Development of an Extensive Linkage Library for Characterization of Carbohydrates.
Article in Analytical chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 43 citations in OpenAlex.
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- Trial
- Characterization and immunomodulatory activity of exopolysaccharides produced byFood science and biotechnology · 2026Article
- Glycoside hydrolase-mediated glucomannan catabolism inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Generation of novel prebiotic oligosaccharide pools from fiber drives biological insight in bacterial glycan metabolism.Applied and environmental microbiology · 2025Article
- A multi-glycomic platform for the analysis of food carbohydrates.Nature protocols · 2024Review
- Dietary fiber monosaccharide content alters gut microbiome composition and fermentation.Applied and environmental microbiology · 2024Article
- Structural Characterization and Anti-Osteoporosis Effects of a Novel Sialoglycopeptide from Tuna Eggs.Marine drugs · 2023Article
- Analysis of Cell Glycogen with Quantitation and Determination of Branching Using Liquid Chromatography-Mass Spectrometry.Analytical chemistry · 2023Article
- Quantitative Bottom-Up Glycomic Analysis of Polysaccharides in Food Matrices Using Liquid Chromatography-Tandem Mass Spectrometry.Analytical chemistry · 2023Article
- Multi-Glycomic Characterization of Fiber from AOAC Methods Defines the Carbohydrate Structures.Journal of agricultural and food chemistry · 2022Article
- An approach for evaluating the effects of dietary fiber polysaccharides on the human gut microbiome and plasma proteome.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A Multidimensional Mass Spectrometry-Based Workflow forJournal of the American Society for Mass Spectrometry · 2021Article
- Combined whole cell wall analysis and streamlined in silico carbohydrate-active enzyme discovery to improve biocatalytic conversion of agricultural crop residues.Biotechnology for biofuels · 2021Review
- Mass spectrometry in clinical glycomics: The path from biomarker identification to clinical implementation.Clinical mass spectrometry (Del Mar, Calif.) · 2020Review
- A nonenzymatic method for cleaving polysaccharides to yield oligosaccharides for structural analysis.Nature communications · 2020Article
- Advancements in Mass Spectrometry-Based Glycomics in Food and Nutritional Science.Mass spectrometry reviewsReview
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The extensive characterization of glycosidic linkages in carbohydrates remains a challenge because of the lack of known standards and limitations in current analytical techniques. This study encompasses the construction of an extensive glycosidic linkage library built from synthesized standards. It includes an improved liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantitation of glycosidic linkages derived from disaccharides, oligosaccharides, and polysaccharides present in complicated matrices. We present a method capable of the simultaneous identification of over 90 unique glycosidic linkages using ultrahigh-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC/QqQ MS) operated in dynamic multiple reaction monitoring (dMRM) mode. To build the library, known monosaccharides commonly found in plants were subjected to partial methylation to yield partially derivatized species representing trisecting, bisecting, linear, and terminal structures. The library includes glycosidic linkage information for three hexoses (glucose, galactose, and mannose), three pentoses (xylose, arabinose, and ribose), two deoxyhexoses (fucose and rhamnose), and two hexuronic acids (glucuronic acid and galacturonic acid). The resulting partially methylated monosaccharides were then labeled with 1-phenyl-3-methyl-5-pyrazolone (PMP) followed by separation and analysis by UHPLC/dMRM MS. Validation of the synthesized standards was performed using disaccharide, oligosaccharide, and polysaccharide standards. Accuracy, reproducibility, and robustness of the method was demonstrated by analysis of xyloglucan (tamarind) and whole carrot root. The synthesized standards represent the most comprehensive group of carbohydrate linkages to date.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.