ArticleAnalytical chemistry2019
Dual-Priming Isothermal Amplification (DAMP) for Highly Sensitive and Specific Molecular Detection with Ultralow Nonspecific Signals.
Article in Analytical chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- A Highly Sensitive and Selective DAMP Assay for the Detection of Bacterial Pathogens Associated With Brain Inflammation.Bio-protocol · 2025Article
- Two-Stage Mixed-Dye-Based Isothermal Amplification with Ribonuclease-Cleavable Enhanced Probes for Dual-Visualization Detection of SARS-CoV-2 Variants of Interest.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- A DAMP-Based Assay for Rapid and Affordable Diagnosis of Bacterial Meningitis Agents:International journal of molecular sciences · 2024Article
- Rapid detection methods for foodborne pathogens based on nucleic acid amplification: Recent advances, remaining challenges, and possible opportunities.Food chemistry. Molecular sciences · 2023Review
- Isothermal nucleic acid amplification and its uses in modern diagnostic technologies.3 Biotech · 2023Review
- Nanotechnology-based diagnostic methods for coronavirus: From nucleic acid extraction to amplification.Biosensors & bioelectronics: X · 2023Review
- Laboratory detection of SARS-CoV-2: A review of the current literature and future perspectives.Heliyon · 2022Review
- pH-EVD: A pH-Paper-Based Extraction and Visual Detection System for Instrument-Free SARS-CoV-2 Diagnostics.Advanced nanobiomed research · 2022Article
- Monolithic, 3D-printed lab-on-disc platform for multiplexed molecular detection of SARS-CoV-2.Sensors and actuators. B, Chemical · 2022Article
- Development of a Rapid and Efficient RPA-CRISPR/Cas12a Assay forFrontiers in microbiology · 2022Article
- Loop-Mediated Isothermal Amplification: From Theory to Practice.Russian journal of bioorganic chemistry · 2022Article
- Improved Strategies for CRISPR-Cas12-based Nucleic Acids Detection.Journal of analysis and testing · 2022Review
- Rapid, Affordable, and Scalable SARS-CoV-2 Detection From Saliva.Journal of biomolecular techniques : JBT · 2021Article
- Sensitive quantitative detection of SARS-CoV-2 in clinical samples using digital warm-start CRISPR assay.Biosensors & bioelectronics · 2021Article
- Point-of-care DNA testing by automatically and sequentially performing extraction, amplification and identification in a closed-type cassette.Sensors and actuators. B, Chemical · 2021Article
- Fabrication of Hard-Soft Microfluidic Devices Using Hybrid 3D Printing.Micromachines · 2020Article
- CRISPR-Cas12a based internal negative control for nonspecific products of exponential rolling circle amplification.Nucleic acids research · 2020Article
- Use of a rapid recombinase-aided amplification assay for Mycoplasma pneumoniae detection.BMC infectious diseases · 2020Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Nucleic acid amplification tests have been widely used in clinical diagnostics, food safety monitoring, and molecular biology. Loop-mediated isothermal amplification (LAMP) is a prevailing nucleic acid isothermal amplification method. It has become a powerful alternative to conventional polymerase chain reaction (PCR) for pathogen detection because of its simplicity, rapidity, and high sensitivity. However, the current LAMP methods, especially LAMP with two loop primers, suffer from undesired nonspecific amplification with strong background signals due to the increasing target sites. This nonspecific amplification substantially reduced the reliability of LAMP and limited its applications in clinical diagnostics. Here, we report a "dual-priming" ("self-priming" and "pairing-priming") isothermal amplification (DAMP) assay for rapid nucleic acid detection with ultralow nonspecific signals. This method takes advantage of the "dual-priming" strand extension strategy by adding two pairing-competition primers and designing unique inner primers, enabling highly sensitive and specific molecular detection. As an application demonstration, the DAMP assay was used to detect HIV-1 DNA/RNA and
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.