ArticleStem cell reports2019
A Chemically Defined Feeder-free System for the Establishment and Maintenance of the Human Naive Pluripotent State.
Article in Stem cell reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Delicate inhibition of NFκB/RELA circuitry facilitates efficient transition towards ground-state pluripotency in human.Nature communications · 2026Article
- Article
- Serum coating enables feeder-free culture of naive human pluripotent stem cells preserving developmental potential.The EMBO journal · 2026Article
- NANOG governs cell metabolism and redox homeostasis in human naïve embryonic stem cells.EMBO reports · 2025Article
- Resistance to naive and formative pluripotency conversion in RSeT human embryonic stem cells.Stem cells (Dayton, Ohio) · 2025Article
- Arp2/3 complex activity enables nuclear YAP for naïve pluripotency of human embryonic stem cells.eLife · 2024Article
- Single-cell 3D genome structure reveals distinct human pluripotent states.Genome biology · 2024Article
- Comprehensive assessment of mRNA isoform detection methods for long-read sequencing data.Nature communications · 2024Article
- Metabolic Quadrivalency in RSeT Human Embryonic Stem Cells.bioRxiv : the preprint server for biology · 2024Article
- Learning Towards Maturation of Defined Feeder-free Pluripotency Culture Systems: Lessons from Conventional Feeder-based Systems.Stem cell reviews and reports · 2024Review
- MetaboLINK is a novel algorithm for unveiling cell-specific metabolic pathways in longitudinal datasets.Frontiers in neuroscience · 2024Article
- Discordance between chromatin accessibility and transcriptional activity during the human primed-to-naïve pluripotency transition process.Cell regeneration (London, England) · 2023Article
- Chemical conversion of human conventional PSCs to TSCs following transient naive gene activation.EMBO reports · 2023Article
- Transcriptomic analysis of feeder-free culture system for maintaining naïve-state pluripotency in human pluripotent stem cells.Stem cell investigation · 2023Article
- Viral Mimicry Response Is Associated With Clinical Outcome in Pleural Mesothelioma.JTO clinical and research reports · 2022Article
- A Comparative Study of Cell Culture Conditions during Conversion from Primed to Naive Human Pluripotent Stem Cells.Biomedicines · 2022Article
- Cell fate roadmap of human primed-to-naive transition reveals preimplantation cell lineage signatures.Nature communications · 2022Article
- Translational and post-translational control of human naïve versus primed pluripotency.iScience · 2022Article
- Large-scale analysis of imprinting in naive human pluripotent stem cells reveals recurrent aberrations and a potential link to FGF signaling.Stem cell reports · 2021Article
- Pluripotent stem cells for the study of early human embryology.Development, growth & differentiation · 2021Review
Corrections and comments
- Erratum issued
Authors and funding
12 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The distinct states of pluripotency in the pre- and post-implantation embryo can be captured in vitro as naive and primed pluripotent stem cell cultures, respectively. The study and application of the naive state remains hampered, particularly in humans, partially due to current culture protocols relying on extraneous undefined factors such as feeders. Here we performed a small-molecule screen to identify compounds that facilitate chemically defined establishment and maintenance of human feeder-independent naive embryonic (FINE) stem cells. The expression profile in genic and repetitive elements of FINE cells resembles the 8-cell-to-morula stage in vivo, and only differs from feeder-dependent naive cells in genes involved in cell-cell/cell-matrix interactions. FINE cells offer several technical advantages, such as increased amenability to transfection and a longer period of genomic stability, compared with feeder-dependent cells. Thus, FINE cells will serve as an accessible and useful system for scientific and translational applications of naïve pluripotent stem cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.