ArticleAnalytica chimica acta2019
Redesigning the T-probe for mass spectrometry analysis of online lysis of non-adherent single cells.
Article in Analytica chimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 35 citations in OpenAlex.
- Developing a Cell Quenching Method to Facilitate Single Cell Mass Spectrometry Metabolomics Studies.JACS Au · 2025Article
- Exploring Single-Probe Single-Cell Mass Spectrometry: Current Trends and Future Directions.Analytical chemistry · 2025Review
- Single Cell mass spectrometry: Towards quantification of small molecules in individual cells.Trends in analytical chemistry : TRAC · 2024Article
- Advances in single-cell metabolomics to unravel cellular heterogeneity in plant biology.Plant physiology · 2023Review
- FACS-assisted single-cell lipidome analysis of phosphatidylcholines and sphingomyelins in cells of different lineages.Journal of lipid research · 2023Article
- Recent developments in ionization techniques for single-cell mass spectrometry.Frontiers in chemistry · 2023Review
- Metabolomics studies of cell-cell interactions using single cell mass spectrometry combined with fluorescence microscopy.Chemical science · 2022Article
- Single cell mass spectrometry studies reveal metabolomic features and potential mechanisms of drug-resistant cancer cell lines.Analytica chimica acta · 2022Article
- Capillary microsampling-based single-cell metabolomics by mass spectrometry and its applications in medicine and drug discovery.Cancer biomarkers : section A of Disease markers · 2022Article
- Single cell metabolomics using mass spectrometry: Techniques and data analysis.Analytica chimica acta · 2021Review
- Chemical Analysis of Single Cells and Organelles.Analytical chemistry · 2021Review
- Combining Mass Spectrometry with Paternò-Büchi Reaction to Determine Double-Bond Positions in Lipids at the Single-Cell Level.Analytical chemistry · 2020Article
- Multimodal Imaging of Amyloid Plaques: Fusion of the Single-Probe Mass Spectrometry Image and Fluorescence Microscopy Image.Analytical chemistry · 2019Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Single cell mass spectrometry (SCMS) allows for molecular analysis of individual cells while avoiding the inevitable drawbacks of using cell lysate prepared from populations of cells. Based on our previous design of the T-probe, a microscale sampling and ionization device for SCMS analysis, we further developed the device to perform online, and real time lysis of non-adherent live single cells for mass spectrometry (MS) analysis at ambient conditions. This redesigned T-probe includes three parts: a sampling probe with a small tip to withdraw a whole cell, a solvent-providing capillary to deliver lysis solution (i.e., acetonitrile), and a nano-ESI emitter in which rapid cell lysis and ionization occur followed by MS analysis. These three components are embedded between two polycarbonate slides and are jointed through a T-junction to form an integrated device. Colon cancer cells (HCT-116) under control and treatment (using anticancer drug irinotecan) conditions were analyzed. We detected a variety of intracellular species, and structural identification of selected ions was conducted using tandem MS (MS
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