ArticleCurrent protocols in microbiology2019
Negative-Stain Transmission Electron Microscopy of Molecular Complexes for Image Analysis by 2D Class Averaging.
Article in Current protocols in microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 52 citations in OpenAlex.
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- Commercial influenza vaccines vary in HA-complex structure and in induction of cross-reactive HA antibodies.Nature communications · 2023Article
- Phase Separation of Rubisco by the Folded SSUL Domains of CcmM in Beta-Carboxysome Biogenesis.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Biophysical Screening Pipeline for Cryo-EM Grid Preparation of Membrane Proteins.Frontiers in molecular biosciences · 2022Article
- Affinity Isolation of Endogenous Saccharomyces Cerevisiae Nuclear Pore Complexes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Purification and Characterization of Human DNA Ligase IIIα Complexes After Expression in Insect Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Nano Milk Protein-Mucilage Complexes: Characterization and Anticancer Effect.Molecules (Basel, Switzerland) · 2021Article
- A Fast Image Alignment Approach for 2D Classification of Cryo-EM Images Using Spectral Clustering.Current issues in molecular biology · 2021Article
- A human apolipoprotein L with detergent-like activity kills intracellular pathogens.Science (New York, N.Y.) · 2021Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Negative-stain transmission electron microscopy (EM) is a technique that has provided nanometer resolution images of macromolecules for about 60 years. Developments in cryo-EM image processing have maximized the information gained from averaging large numbers of particles. These developments can now be applied back to negative-stain image analysis to ascertain domain level molecular structure (10 to 20 Å) more quickly and efficiently than possible by atomic resolution cryo-EM. Using uranyl acetate stained molecular complexes of influenza hemagglutinin bound to Fab 441D6, we describe a simple and efficient means to collect several hundred micrographs with SerialEM. Using RELION, we illustrate how tens of thousands of complexes can be auto-picked and classified to accurately describe the domain level topology of this unconventional hemagglutinin head-domain epitope. By comparing to the cryo-EM density map of the same complex, we show that questions about epitope mapping and conformational heterogeneity can readily be answered by this negative-stain method. © 2019 The Authors.
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