ArticleCurrent protocols in microbiology2019
High-Throughput Gene Replacement in Aspergillus fumigatus.
Article in Current protocols in microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 64 citations in OpenAlex.
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- Barcoded mutant library enables high-throughput functional genomics in a filamentous fungus.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- The BRCT domain enhances DNA binding and catalytic efficiency of fungal PARPs.Nature communications · 2026Article
- Aneuploidy promotes transient stress adaptation and metabolic flexibility in the human fungal pathogen Aspergillus fumigatus.Current biology : CB · 2026Article
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- Genome-wide discovery and phenotyping of non-coding transcripts in A. fumigatus reveals lncRNAs with a role in antifungal drug sensitivity.Nature communications · 2026Article
- Oxidoreductase geneMicrobiology spectrum · 2026Article
- The sulfur-related metabolic status ofVirulence · 2025Article
- Construction of a randomly barcoded insertional mutant library in the filamentous fungusbioRxiv : the preprint server for biology · 2025Article
- In vitro combination effects and mechanisms of Revaprazan with Triazole antifungal drugs on Aspergillus.BMC microbiology · 2025Article
- Genetic, structural, and functional characterization of allomelanin from black yeast Exophiala viscosa, a chassis for fungal melanin production.Applied microbiology and biotechnology · 2025Article
- Whole-chromosome duplications drive antimicrobial resistance inbioRxiv : the preprint server for biology · 2025Article
- Agricultural SDHIs Induce Azole Resistance in Aspergillus fumigatus via Mitochondrial Sdh1 Suppression.Mycopathologia · 2025Article
- First Report ofPathogens (Basel, Switzerland) · 2025Article
- Extracting Protoplasts from Filamentous Fungi Using Extralyse, An Enzyme Used in the Wine Industry.Current protocols · 2025Article
- Considerations for Domestication of Novel Strains of Filamentous Fungi.ACS synthetic biology · 2025Review
- Exploring the role and mechanisms of theMycology · 2025Article
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- Shining a light on the impact of antifungals onAntimicrobial agents and chemotherapy · 2024Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Aspergillus fumigatus is a human pathogen and the principal etiologic agent of invasive and chronic aspergillosis leading to several hundreds of thousands of deaths every year. Very few antifungals are available to treat infections caused by A. fumigatus, and resistance is developing to those we have. Our understanding of the molecular mechanisms that drive pathogenicity and drug resistance have been hampered by the lack of large mutant collections, which limits our ability to perform functional genomics analysis. Here we present a high-throughput gene knockout method that combines a highly reproducible fusion PCR method to enable generation of gene replacement cassettes with a multiwell format transformation procedure. This process can be used to generate 96 null mutants within 5 days by a single person at a cost of less than £18 ($24) per mutant and is being employed in our laboratory to generate a barcoded genome-wide knockout library in A. fumigatus. © 2019 The Authors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.