ArticleCurrent protocols in microbiology2019
Fast and Reliable PCR Amplification from Aspergillus fumigatus Spore Suspension Without Traditional DNA Extraction.
Article in Current protocols in microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 39 citations in OpenAlex.
- Genome-wide discovery and phenotyping of non-coding transcripts in A. fumigatus reveals lncRNAs with a role in antifungal drug sensitivity.Nature communications · 2026Article
- Regulation of extracellular vesicles for protein secretion inMicrobial cell (Graz, Austria) · 2026Article
- High-Throughput Culture and DNA Isolation Methods for Aspergillus fumigatus.Current protocols · 2025Article
- Land use drives drug resistance in an airborne human fungal pathogen.The ISME journal · 2025Article
- Optimizing a modified cetyltrimethylammonium bromide protocol for fungal DNA extraction: Insights from multilocus gene amplification.Open life sciences · 2025Article
- An innovative high-throughput genome releaser for rapid and efficient PCR screening.Frontiers in bioengineering and biotechnology · 2025Article
- Article
- Reverse-transcriptase real-time PCR in the diagnostic strategy for invasive infections caused byJournal of clinical microbiology · 2024Article
- Development of a whole-cell SELEX process to select species-specific aptamers against Aspergillus niger.Fungal biology and biotechnology · 2024Article
- Catching some air: a method to spatially quantify aerial triazole resistance inApplied and environmental microbiology · 2024Article
- Universal digital high-resolution melting for the detection of pulmonary mold infections.Journal of clinical microbiology · 2024Article
- Improved gene editing and fluorescent-protein tagging inWellcome open research · 2024Article
- Universal Digital High Resolution Melt for the detection of pulmonary mold infections.bioRxiv : the preprint server for biology · 2023Article
- The human fungal pathogen Aspergillus fumigatus can produce the highest known number of meiotic crossovers.PLoS biology · 2023Article
- Rapid and robust squashed spore/colony PCR of industrially important fungi.Fungal biology and biotechnology · 2023Article
- VdERG2 was involved in ergosterol biosynthesis, nutritional differentiation and virulence of Verticillium dahliae.Current genetics · 2023Article
- Rapid Detection ofInternational journal of molecular sciences · 2022Article
- Telomere-to-telomere genome sequence of the model mould pathogen Aspergillus fumigatus.Nature communications · 2022Article
- Incidence of Coffee Leaf Rust in Vietnam, Possible Original Sources and Subsequent Pathways of Migration.Frontiers in plant science · 2022Article
- Inhibition of Quorum Sensing and Virulence Factors ofAntibiotics (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Aspergillus fumigatus is an opportunistic human pathogenic mold. DNA extraction from this fungus is usually performed by mechanical perturbation of cells, as it possesses a rigid and complex cell wall. While this is not problematic for single isolates, it can be time consuming for large numbers of strains if using traditional DNA extraction procedures. Therefore, in this article we describe a fast and efficient thermal-shock method to release DNA from spores of A. fumigatus and other filamentous fungi without the need for complex extraction methods. This is especially important for high-throughput PCR analyses of mutants in 96- or 384-well formats in a very short period of time without any concern about sample cross-contamination. This method is currently being used to validate the protein-coding gene and non-coding RNA knockout libraries in A. fumigatus generated in our laboratory, and could be used in the future for diagnostics purposes. © 2019 The Authors.
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