ArticleHeliyon2019
Evaluation of vacuum washing in the removal of SDS from decellularized bovine pericardium: method and device description.
Article in Heliyon, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 49 citations in OpenAlex.
- Initial attachment, viability, proliferation, and migration of osteoblast-like SaOS-2 cells on two resorbable xenogeneic membranes for guided tissue regeneration: ab in vitro experimental study.Restorative dentistry & endodontics · 2026Article
- Cryopreservation preserves the morpho-structural and mechanical integrity of human decellularized tracheas and supports a reduced immunogenic profile.Frontiers in bioengineering and biotechnology · 2026Article
- Decellularization of camel corneas for tissue engineering applications.International ophthalmology · 2025Article
- Decellularized Liver Matrices for Expanding the Donor Pool-An Evaluation of Existing Protocols and Future Trends.Biomolecules · 2025Review
- Recent advances in pericardium extracellular matrix for tissue regeneration, along with a short insight into artificial intelligence.Frontiers in medical technology · 2025Review
- Advances in xenogeneic donor decellularized organs: A review on studies with sheep and porcine-derived heart valves.Bioengineering & translational medicine · 2024Review
- Histological evaluation of decellularization of freeze dried and chemically treated indigenously prepared bovine pericardium membrane.Cell and tissue banking · 2024Article
- Recent advances in hydrogels applications for tissue engineering and clinical trials.Regenerative therapy · 2024Review
- Effects of Various Decellularization Methods for the Development of Decellularized Extracellular Matrix from Tilapia (International journal of biomaterials · 2024Article
- Decellularization Techniques for Tissue Engineering: Towards Replicating Native Extracellular Matrix Architecture in Liver Regeneration.Journal of functional biomaterials · 2023Review
- Evaluation of Structural Viability of Porcine Tracheal Scaffolds after 3 and 6 Months of Storage under Three Different Protocols.Bioengineering (Basel, Switzerland) · 2023Article
- Preparation of extracellular matrix of fish swim bladders by decellularization with supercritical carbon dioxide.Bioresources and bioprocessing · 2023Article
- Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine.Biomaterials research · 2023Review
- Reconstruction of the human nipple-areolar complex: a tissue engineering approach.Frontiers in bioengineering and biotechnology · 2023Article
- Characterization of Decellularized Extracellular Matrix from Milkfish (Biomimetics (Basel, Switzerland) · 2022Article
- An Effective Method for Decellularization of Human Foreskin: Implications for Skin Regeneration in Small Wounds.Cell journal · 2022Article
- Cell loaded hydrogel containing Ag-doped bioactive glass-ceramic nanoparticles as skin substitute: Antibacterial properties, immune response, and scarless cutaneous wound regeneration.Bioengineering & translational medicine · 2022Article
- Human-Origin iPSC-Based Recellularization of Decellularized Whole Rat Livers.Bioengineering (Basel, Switzerland) · 2022Article
- Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering.Bioactive materials · 2022Review
- Decellularized Tissues for Wound Healing: Towards Closing the Gap Between Scaffold Design and Effective Extracellular Matrix Remodeling.Frontiers in bioengineering and biotechnology · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsThe aim of this study was to present a new method for removing Sodium dodecyl sulfate (SDS) detergent from decellularized bovine pericardium using vacuum. MATERIALS AND
methodsThe cows' pericardia were collected and decellularized. The samples were incubated with SDS1% for 48 h at 40 °C. To perform vacuum washing (VW: negative pressure was used to wash and remove detergents), every decellularized tissue was cut in 75mm diameter and fixed via a stainless-steel ring with 60mm diameter in the center of filtration Buchner Funnel which was connected to glass filtration flask The system was connected to a vacuum pump by a hose, and a negative pressure of -100 mmHg was applied for 15 min. Then, the samples were shaken and washed at 40-rpm in 100 ml of distilled water for 45 min. This process was repeated for samples of each group (6 times for sample VW6h, 12 times for sample VW12h, and 24 times for sample VW24h). At the end of every cycle, the effluent was collected to take a sample for SDS measurement. The normal washing (NW) group containing distilled water (NWd) and PBS (Phosphate buffered saline) (NWp) were used to wash and remove detergents. SDS measurements, MTT Assay, histological and tensile test, to compare two methods were used.
resultsThe highest SDS in the effluent was in groups VW12h and VW24h (P ≤ 0.001) and the lowest residual SDS in scaffold was in two groups of VW12h and VW24h (P ≤ 0.001). MTT assay showed that cell survival in the VW12h and VW24h groups was higher than other groups and there' was no significant difference between cell survival in the VW12h and VW24h groups. Histological study showed destruction of tissue in the VW24h group. The results of the tensile test were shown that the native group had the highest module and the lowest amount was the VW24h sample which was reported with P ≤ 0.001 significance for all groups.
conclusionVW12h can be used as an effective method for SDS removal from decellularized pericardium which morphologically demonstrated a good structure in ECM.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.