Evidence map›Paper›PMID 31515946›Full record

ArticleJournal of cellular and molecular medicine2019

Biphasic effects of autophagy on decompression bubble-induced endothelial injury.

Mengmeng Wang, Kun Zhang, Shaojie Nie, Guoyang Huang, Hongjie Yi, Chunyang He, Peter Buzzacott, Weigang Xu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular and molecular medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Mengmeng WangDepartment of Diving and Hyperbaric Medicine, Naval Medical University, Shanghai, China.ORCID 0000-0002-6334-202X
Kun ZhangDepartment of Diving and Hyperbaric Medicine, Naval Medical University, Shanghai, China.
Shaojie NieAdministration Office for Undergraduates, Naval Medical University, Shanghai, China.
Guoyang HuangDepartment of Diving and Hyperbaric Medicine, Naval Medical University, Shanghai, China.
Hongjie YiDepartment of Hyperbaric Oxygen, Changhai Hospital, Shanghai, China.
Chunyang HeDepartment of Hyperbaric Oxygen, General Hospital in Western Theater of Operations, Chengdu, China.
Peter BuzzacottSchool of Sports Science, Exercise and Health, The University of Western Australia, Crawley, Perth, Australia.
Weigang XuDepartment of Diving and Hyperbaric Medicine, Naval Medical University, Shanghai, China.
Chengdu Military General Hospital · CNSecond Military Medical University · CNThe University of Western Australia · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Endothelial dysfunction induced by bubbles plays an important role in decompression sickness (DCS), but the mechanism of which has not been clear. The present study was to investigate the role of autophagy in bubble-induced endothelial injury. Human umbilical vein endothelial cells (HUVECs) were treated with bubbles, autophagy markers and endothelial injury indices were determined, and relationship strengths were quantified. Effects of autophagy inhibitor 3-methyladenine (3-MA) were observed. Bubble contact for 1, 5, 10, 20 or 30 minutes induced significant autophagy with increases in LC3-II/I ratio and Beclin-1, and a decrease in P62, which correlated with bubble contact duration. Apoptosis rate, cytochrome C and cleaved caspase-3 increased, and cell viability decreased following bubble contact for 10, 20 or 30 minutes, but not for 1 or 5 minutes. Injuries in HUVECs were correlated with LC3-II/I ratio and partially reversed by 3-MA in 10, 20 or 30 minutes contact, but worsened in 1 or 5 minutes. Bubble pre-conditioning for 1 minutes resulted in increased cell viability and decreased apoptosis rate compared with no pre-conditioning, and 30-minutes pre-conditioning induced opposing changes, all of which were inhibited by 3-MA. In conclusion, autophagy was involved and played a biphasic role in bubble-induced endothelial injury.

Indexed as

AdenineApoptosisAutophagosomesAutophagyBeclin-1Caspase 3Cell SurvivalCytochromes cDecompression SicknessEndotheliumHumansHuman Umbilical Vein Endothelial CellsMicroscopy, Electron, TransmissionMicrotubule-Associated ProteinsRNA-Binding ProteinsTime Factors3-methyladenineAdenineBeclin-1BECN1 protein, humanCASP3 protein, humanCaspase 3Cytochromes cMAP1LC3A protein, humanMAP1LC3B protein, humanMicrotubule-Associated ProteinsP62 protein, humanRNA-Binding Proteinsautophagybubbledecompression sicknessendothelial cell

Identifiers

PMID31515946
PMCPMC6850936
OpenAlexW2973163596

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.