ArticleViruses2019
A Deep-Sequencing Workflow for the Fast and Efficient Generation of High-Quality African Swine Fever Virus Whole-Genome Sequences.
Article in Viruses, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- Genome sequencing reveals variation of African swine fever virus in Nigerian outbreaks and identification of two major West African viral lineages.Microbial genomics · 2026Article
- Article
- African swine fever virus I177L induces host inflammatory responses by facilitating the TRAF6-TAK1 axis and NLRP3 inflammasome assembly.Journal of virology · 2025Article
- Genome-Wide Approach Identifies Natural Large-Fragment Deletion in ASFV Strains Circulating in Italy During 2023.Pathogens (Basel, Switzerland) · 2025Article
- Unique Nucleotide Polymorphism of African Swine Fever Virus Circulating in East Asia and Central Russia.Viruses · 2024Article
- A Novel and Rapid Selective Viral Genome Amplification and Sequencing Method for African Swine Fever Virus.Viruses · 2024Article
- Article
- A Standardized Pipeline for Assembly and Annotation of African Swine Fever Virus Genome.Viruses · 2024Article
- The Applications of Nanopore Sequencing Technology in Animal and Human Virus Research.Viruses · 2024Review
- Generation of High-Quality African Swine Fever Virus Complete Genome from Field Samples by Next-Generation Sequencing.Viruses · 2024Article
- A Deep Sequencing Strategy for Investigation of Virus Variants within African Swine Fever Virus-Infected Pigs.Pathogens (Basel, Switzerland) · 2024Article
- Article
- African swine fever virus - variants on the rise.Emerging microbes & infections · 2023Article
- Emergence and Prevalence of an African Swine Fever Virus Variant in Wild Boar Populations in South Korea from 2019 to 2022.Viruses · 2023Article
- Tracing the Origin of Genotype II African Swine Fever Virus in China by Genomic Epidemiology Analysis.Transboundary and emerging diseases · 2023Article
- Coding-Complete Genome Sequence of an African Swine Fever Virus from an Outbreak in 2021 among Domestic Pigs in Pangasinan, Philippines.Microbiology resource announcements · 2022Article
- Genome-Wide Diversity Analysis of African Swine Fever Virus Based on a Curated Dataset.Animals : an open access journal from MDPI · 2022Article
- Nanopore sequencing of a monkeypox virus strain isolated from a pustular lesion in the Central African Republic.Scientific reports · 2022Article
- African Swine Fever Virus and Host Response: Transcriptome Profiling of the Georgia 2007/1 Strain and Porcine Macrophages.Journal of virology · 2022Article
- Low-host double MDA workflow for uncultured ASFV positive blood and serum sample sequencing.Frontiers in veterinary science · 2022Article
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13 authors.
Funding
Abstract
African swine fever (ASF) is a severe disease of suids caused by African swine fever virus (ASFV). Its dsDNA genome (170-194 kbp) is scattered with homopolymers and repeats as well as inverted-terminal-repeats (ITR), which hamper whole-genome sequencing. To date, only a few genome sequences have been published and only for some are data on sequence quality available enabling in-depth investigations. Especially in Europe and Asia, where ASFV has continuously spread since its introduction into Georgia in 2007, a very low genetic variability of the circulating ASFV-strains was reported. Therefore, only whole-genome sequences can serve as a basis for detailed virus comparisons. Here, we report an effective workflow, combining target enrichment, Illumina and Nanopore sequencing for ASFV whole-genome sequencing. Following this approach, we generated an improved high-quality ASFV Georgia 2007/1 whole-genome sequence leading to the correction of 71 sequencing errors and the addition of 956 and 231 bp at the respective ITRs. This genome, derived from the primary outbreak in 2007, can now serve as a reference for future whole-genome analyses of related ASFV strains and molecular approaches. Using both workflow and the reference genome, we generated the first ASFV-whole-genome sequence from Moldova, expanding the sequence knowledge from Eastern Europe.
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