ArticleMicroorganisms2019
DNA- and RNA- Derived Fungal Communities in Subsurface Aquifers Only Partly Overlap but React Similarly to Environmental Factors.
Article in Microorganisms, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed.
- Invasive earthworms shift soil microbial community structure in northern North American forest ecosystems.iScience · 2024Article
- Article
- Temporal dynamics of total and active root-associated diazotrophic communities in field-grown rice.Frontiers in microbiology · 2022Article
- Tree diversity and soil chemical properties drive the linkages between soil microbial community and ecosystem functioning.ISME communications · 2021Article
- Site and Bioenergy Cropping System Similarly Affect Distinct Live and Total Soil Microbial Communities.Frontiers in microbiology · 2021Article
- Targeting the Active Rhizosphere Microbiome ofFrontiers in microbiology · 2021Article
- Root-Associated Mycobiome Differentiate between Habitats Supporting Production of Different Truffle Species in Serbian Riparian Forests.Microorganisms · 2020Article
- Comparing the Influence of Assembly Processes Governing Bacterial Community Succession Based on DNA and RNA Data.Microorganisms · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Recent advances in high-throughput sequencing (HTS) technologies have revolutionized our understanding of microbial diversity and composition in relation to their environment. HTS-based characterization of metabolically active (RNA-derived) and total (DNA-derived) fungal communities in different terrestrial habitats has revealed profound differences in both richness and community compositions. However, such DNA- and RNA-based HTS comparisons are widely missing for fungal communities of groundwater aquifers in the terrestrial biogeosphere. Therefore, in this study, we extracted DNA and RNA from groundwater samples of two pristine aquifers in the Hainich CZE and employed paired-end Illumina sequencing of the fungal nuclear ribosomal internal transcribed spacer 2 (ITS2) region to comprehensively test difference/similarities in the "total" and "active" fungal communities. We found no significant differences in the species richness between the DNA- and RNA-derived fungal communities, but the relative abundances of various fungal operational taxonomic units (OTUs) appeared to differ. We also found the same set of environmental parameters to shape the "total" and "active" fungal communities in the targeted aquifers. Furthermore, our comparison also underlined that about 30%-40% of the fungal OTUs were only detected in RNA-derived communities. This implies that the active fungal communities analyzed by HTS methods in the subsurface aquifers are actually not a subset of supposedly total fungal communities. In general, our study highlights the importance of differentiating the potential (DNA-derived) and expressed (RNA-derived) members of the fungal communities in aquatic ecosystems.
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