ArticlePloS one2019
Comparison between Aptima Assays (Hologic) and the Allplex STI Essential Assay (Seegene) for the diagnosis of Sexually transmitted infections.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Molecular Diagnosis ofJournal of tropical medicine · 2026Review
- Diagnostic accuracy of real-time polymerase chain reaction assay for the detection ofAfrican journal of laboratory medicine · 2025Review
- Detection ofAntibiotics (Basel, Switzerland) · 2024Article
- Prevalence of sexually transmitted infections and associated risk factors among female sex workers in Guinea-Bissau.Sexually transmitted infections · 2024Article
- Detection of Trichomonas vaginalis by Allplex™ STI Essential Assay (Seegene) in clinical samples from the Brazilian public health system users.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Article
- Estimating the prevalence of two or more diseases using outcomes from multiplex group testing.Biometrical journal. Biometrische Zeitschrift · 2023Article
- A retrospective evaluation of the Euroarray STI-11 multiplex system for the detection of eight STI causing agents.Scientific reports · 2023Article
- Latest Advances in Laboratory Detection of Mycoplasma genitalium.Journal of clinical microbiology · 2023Review
- Pooling Rectal, Pharyngeal, and Urine Samples to DetectOpen forum infectious diseases · 2022Article
- Monitoring Sexually Transmitted Infections in Cervicovaginal Exfoliative Samples in Mexican Women.Pathogens (Basel, Switzerland) · 2021Article
- Review
- A profile of the binx healthExpert review of molecular diagnostics · 2021Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sexually transmitted infections (STIs) remain a worldwide problem and a severe threat to public health. The purpose of this study was to compare Aptima® Assays (Hologic®) and the Allplex™ STI Essential Assay (Seegene®) for the simultaneous detection of Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis and Mycoplasma genitalium in clinical practice. The Aptima® assays (Hologic®) are based on a transcription-mediated amplification (TMA) method. The Allplex™ STI Essential assay (Seegene®) is based on a multiplex Real-Time PCR (RT-PCR) method. A total of 622 clinical samples from different anatomical sites were tested using both methods. A total of 88 (14.1%) and 66 (10.6%) positive samples were found for any of the TMA assays used and for the RT-PCR assay, respectively. Aptima® assays showed a slightly higher rate of positive results for all pathogens except for T. vaginalis, the results of which were similar to those obtained with Allplex™. The most commonly detected pathogen was C. trachomatis (37 samples; 5.9% using TMA assays) and the anatomical site with the highest prevalence of microorganisms was a non-urogenital site, the pharynx (27 positive samples; 4.3%). Using the Aptima® assays as reference method, the comparison showed that the average specificity of multiplex RT-PCR was 100.0% for the four pathogens. However an average sensitivity of 74.5% was observed, showing 95.2% (CI95%; 93.6-96.9) of overall concordance (κ = 0.80). In conclusion, the Aptima® assays show a higher sensitivity on a wide range of sample types compared to the Allplex™ assay.
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