Evidence map›Paper›PMID 31512048›Full record

ArticleJournal of assisted reproduction and genetics2019

Novel association between apoptotic sperm biomarkers with seminal biochemical parameters and acetylcholinesterase activity in patients with teratozoospermia.

Oumaima Ammar, Meriem Mehdi, Oumayma Tekeya, Fadoua Neffati, Zohra Haouas

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.9field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Oumaima AmmarLaboratory of Histology Embryology and Cytogenetics (LR 40 ES 18), Faculty of Medicine, University of Monastir, Avicenne Street, 5019, Monastir, Tunisia. ammaroumayma2014@gmail.com.ORCID http://orcid.org/0000-0003-0933-6662
Meriem MehdiLaboratory of Histology Embryology and Cytogenetics (LR 40 ES 18), Faculty of Medicine, University of Monastir, Avicenne Street, 5019, Monastir, Tunisia.
Oumayma TekeyaLaboratory of Histology Embryology and Cytogenetics (LR 40 ES 18), Faculty of Medicine, University of Monastir, Avicenne Street, 5019, Monastir, Tunisia.
Fadoua NeffatiLaboratory of Biochemistry and Toxicology, Fattouma Bourguiba University Teaching Hospital of Monastir, Monastir, Tunisia.
Zohra HaouasLaboratory of Histology Embryology and Cytogenetics (LR 40 ES 18), Faculty of Medicine, University of Monastir, Avicenne Street, 5019, Monastir, Tunisia.
University of Monastir · TNHospital Fatuma Bourguiba Monastir · TN

Funding

Ministère de l'Enseignement Supérieur, de la Recherche Scientifique et des Technologies de l'Information et de la Communication This work was supported by funds allocated to the Research laboratory of Histology and Genetic LR40ES18 by the Ministère Tunisien de l'Enseingement Supérieur et de la Recherché Scientifique.
6 · The paper itself

Abstract

objectiveWe aimed to determine whether the dysfunction of physiological apoptosis and specific seminal biochemical parameters could be associated with male infertility and sperm morphological defects. STUDY

designEjaculated sperm samples from sixty patients with isolated teratozoospermia and thirty fertile donors were analyzed. The proportion of both viable and dead spermatozoa expressing activated caspases was detected by fluorescence microscopy through the use of different specific carboxyfluorescein-labeled caspase inhibitors FLICA. The different stages of apoptosis in human were qualitatively and quantitatively determined by using the AO/EB fluorescent staining method. The levels of the seminal biochemical parameters (acetylcholinesterase (AChE), lactate dehydrogenase (LDH), creatine phosphokinase (CK), iron (Fe), calcium (Ca), and phosphorus (P)) were evaluated spectrophotometrically.

resultsPatients with teratozoospermia showed significantly higher proportions of dead and live spermatozoa with activated caspases and spermatozoa in the late stage of apoptosis when compared to controls. Among the different studied biochemical seminal parameters, the rates of acetylcholinesterase activity, creatine phosphokinase, iron, and calcium were significantly increased in the patient group. However, the rate of phosphorus was significantly decreased. Interestingly, significant relationships were found between the studied biochemical and apoptotic biomarkers and the rates of atypical sperm forms with the incidences of head, mid-piece, and tail abnormalities. Furthermore, positive correlations were found between P, AChE, Fe, CK, and LDH with apoptotic markers.

conclusionsThese results emphasize the impact of apoptosis in the pathophysiology of teratozoospermia and suggest that seminal biochemical disturbance may arise such damage.

Indexed as

AcetylcholinesteraseAdultApoptosisBiomarkersFertilityHumansInfertility, MaleMaleProspective StudiesSemenSpermatozoaSperm MotilityTeratozoospermiaAcetylcholinesteraseBiomarkersApoptotic sperm biomarkersPolycaspasesSeminal biochemical parametersTeratozoospermia

Identifiers

PMID31512048
PMCPMC6885487
OpenAlexW2973033983

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.