Evidence map›Paper›PMID 31511839›Full record

ArticleTranslational medicine of aging2019

An inexpensive microscopy system for microfluidic studies in budding yeast.

Kenneth L Chen, Toby N Ven, Matthew M Crane, Dexter E Chen, Yen-Chi Feng, Nozomi Suzuki, Adam E Russell, Diogo de Moraes, Matt Kaeberlein

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Translational medicine of aging, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Kenneth L ChenDepartment of Pathology, School of Medicine, University of Washington, Seattle, WA, USA.
Toby N VenDepartment of Pathology, School of Medicine, University of Washington, Seattle, WA, USA.
Matthew M CraneDepartment of Pathology, School of Medicine, University of Washington, Seattle, WA, USA.
Dexter E ChenDepartment of Pathology, School of Medicine, University of Washington, Seattle, WA, USA.
Yen-Chi FengDepartment of Pathology, School of Medicine, University of Washington, Seattle, WA, USA.
Nozomi SuzukiDepartment of Pathology, School of Medicine, University of Washington, Seattle, WA, USA.
Adam E RussellDepartment of Pathology, School of Medicine, University of Washington, Seattle, WA, USA.
Diogo de MoraesDepartment of Pathology, School of Medicine, University of Washington, Seattle, WA, USA.
Matt KaeberleinDepartment of Pathology, School of Medicine, University of Washington, Seattle, WA, USA.

Funding

SPECIFICITY OF OXYGEN DNA DAMAGE AND MUTAGENESISP01AG001751 · NIA · UNIVERSITY OF WASHINGTON · PI RABINOVITCH, PETER S · 1985 to 2021
$29.6M
University of Washington Nathan Shock Center of Excellence in the Basic Biology of AgingP30AG013280 · NIA · UNIVERSITY OF WASHINGTON · PI Su-In Lee · 1995 to 2026
$27.1M
GENETIC APPROACHES TO AGING RESEARCHT32AG000057 · NIA · UNIVERSITY OF WASHINGTON · PI RABINOVITCH, PETER S · 1985 to 2019
$14.2M
Emergent properties of signaling network degradation that mediate homeostatic failure during agingR01AG056359 · NIA · UNIVERSITY OF WASHINGTON · PI KAEBERLEIN, MATT, VILLEN, JUDIT · 2017 to 2021
$2.9M
Whole-lifespan monitoring of yeast healthF30AG052225 · NIA · UNIVERSITY OF WASHINGTON · PI CHEN, KENNETH · 2016 to 2019
$179k
NIA NIH HHS F30 AG052225NIA NIH HHS P01 AG001751NIA NIH HHS P30 AG013280NIA NIH HHS R01 AG056359NIA NIH HHS T32 AG000057
6 · The paper itself

Abstract

Recently, microfluidic technologies have been developed to allow higher throughput collection of yeast replicative lifespan data. Adoption of these devices has been limited, in part, due to the high cost of the motorized microscopy instrumentation from mainline manufacturers. Inspired by recent development of open source microscopy hardware and software, we developed minimal-cost hardware attachments to provide long-term focus stabilization for lower-cost microscopes and open source software to manage concurrent time-lapse image acquisition from multiple microscopes. We hope that these tools will help spur the wider adoption of microfluidic technologies for the study of aging in yeast.

Indexed as

3D printingArduinoLifespanMicrodissectionMicrofluidicsMicroscopyReplicative agingYeast

Identifiers

PMID31511839
PMCPMC6738973

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.