ArticleScientific reports2019
An Experimental Approach to Rigorously Assess Paneth Cell α-Defensin (Defa) mRNA Expression in C57BL/6 Mice.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Intelectin-2 is a broad-spectrum antimicrobial lectin.Nature communications · 2026Article
- Disentangling the impact of obesity, diet, host factors, and microbiota on small intestinal antimicrobial peptide expression.Gut microbes · 2025Article
- Loss of sialic acid side-chainProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Identification and Characterization of Multiple Paneth Cell Types in the Mouse Small Intestine.Cells · 2024Article
- Balancing Act of the Intestinal Antimicrobial Proteins on Gut Microbiota and Health.Journal of microbiology (Seoul, Korea) · 2024Review
- Treatment of peanut allergy and colitis in mice via the intestinal release of butyrate from polymeric micelles.Nature biomedical engineering · 2023Article
- Toxoplasma gondii Infection Decreases Intestinal 5-Lipoxygenase Expression, while Exogenous LTBInfection and immunity · 2022Article
- Human intelectin-2 (ITLN2) is selectively expressed by secretory Paneth cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Epithelial colonization by gut dendritic cells promotes their functional diversification.Immunity · 2022Article
- Characterization of an intelectin-1 (Frontiers in immunology · 2022Article
- A potent HNF4α agonist reveals that HNF4α controls genes important in inflammatory bowel disease and Paneth cells.PloS one · 2022Article
- Article
- Extensive variation in the intelectin gene family in laboratory and wild mouse strains.Scientific reports · 2021Article
- Human intelectin-1 (ITLN1) genetic variation and intestinal expression.Scientific reports · 2021Article
- Astaxanthin Alleviates Ochratoxin A-Induced Cecum Injury and Inflammation in Mice by Regulating the Diversity of Cecal Microbiota and TLR4/MyD88/NF-Oxidative medicine and cellular longevity · 2021Article
- Expression and Localization of Paneth Cells and Their α-Defensins in the Small Intestine of Adult Mouse.Frontiers in immunology · 2020Article
- Does an Apple a Day Also Keep the Microbes Away? The Interplay Between Diet, Microbiota, and Host Defense Peptides at the Intestinal Mucosal Barrier.Frontiers in immunology · 2020Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Abundant evidence from many laboratories supports the premise that α-defensin peptides secreted from Paneth cells are key mediators of host-microbe interactions in the small intestine that contribute to host defense and homeostasis. α-defensins are among the most highly expressed antimicrobial peptides at this mucosal surface in many mammals, including humans and mice; however, there is striking variation among species in the number and primary structure of α-defensin paralogs. Studies of these biomolecules in vivo are further complicated by striking variations between laboratory mouse strains. Herein, we report an experimental approach to determine with precision and specificity expression levels of α-defensin (Defa) mRNA in the small intestine of C57BL/6 mice through an optimized set of oligonucleotide primers for qRT-PCR assays and cloned cDNA plasmids corresponding to the Defa paralogs. This approach demonstrated marked differences in α-defensin expression in C57BL/6 mice with respect to proximal/distal anatomical location and developmental stage, which have not been described previously. These data underscore the importance of careful attention to method (primer choice, proximal vs. distal location, and developmental stage) in analysis of antimicrobial peptide expression and their impact.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.