Evidence map›Paper›PMID 31511005›Full record

ArticleJournal of orthopaedic surgery and research2019

Icariin alleviates osteoarthritis by inhibiting NLRP3-mediated pyroptosis.

Yan Zu, Yue Mu, Qiang Li, Shu-Ting Zhang, Hong-Juan Yan

Registry-linked trialOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of orthopaedic surgery and research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04395547 (A Randomized, Double-blind, Placebo-controlled, Parallel Study to Investigate the Safety and Efficacy of JointAlive™ on Improving Knee Joint Function in Adults With Mild to Moderate Knee Osteoarthritis), which is not on this map. Cited by 114 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
114citing papers in PubMed, 2 pooled it
7.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT04395547 nacompletednot on this mapstarted 2020, after this paper: background citation

A Randomized, Double-blind, Placebo-controlled, Parallel Study to Investigate the Safety and Efficacy of JointAlive™ on Improving Knee Joint Function in Adults With Mild to Moderate Knee Osteoarthritis

TypeinterventionalSponsorChenland Nutritionals Inc.Ran2020 to 2022Enrolled72ConditionsOsteo Arthritis KneeArmsJointAlive™, Placebo
3 · Its place in the literature

Who cites it

114 citing papers in PubMed, 2 syntheses or guidelines pooled it, 192 citations in OpenAlex.

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54 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Yan ZuSchool of Mechanical and Material Engineering, North China University of Technology, No.5, Jinyuanzhuang Road, Shijingshan District, Beijing, 100144, People's Republic of China. zuyan@ncut.edu.cn.ORCID http://orcid.org/0000-0001-9162-457X
Yue MuDepartment of Stomatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, 100730, People's Republic of China.
Qiang LiSchool of Mechanical and Material Engineering, North China University of Technology, No.5, Jinyuanzhuang Road, Shijingshan District, Beijing, 100144, People's Republic of China.
Shu-Ting ZhangSchool of Mechanical and Material Engineering, North China University of Technology, No.5, Jinyuanzhuang Road, Shijingshan District, Beijing, 100144, People's Republic of China.
Hong-Juan YanSchool of Mechanical and Material Engineering, North China University of Technology, No.5, Jinyuanzhuang Road, Shijingshan District, Beijing, 100144, People's Republic of China.
North China University of Technology · CNChinese Academy of Medical Sciences & Peking Union Medical College · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundOsteoarthritis (OA) is the common chronic degenerative joint bone disease that is mainly featured by joint stiffness and cartilage degradation. Icariin (ICA), an extract from Epimedium, has been preliminarily proven to show anti-osteoporotic and anti-inflammatory effects in OA. However, the underlying mechanisms of ICA on chondrocytes need to be elucidated.

methodsLPS-treated chondrocytes and monosodium iodoacetate (MIA)-treated Wistar rats were used as models of OA in vitro and in vivo, respectively. LDH and MTT assays were performed to detect cytotoxicity and cell viability. The expression levels of NLRP3, IL-1β, IL-18, MMP-1, MMP-13, and collagen II were detected by qRT-PCR and Western blotting. The release levels of IL-1β and IL-18 were detected by ELISA assay. Caspase-1 activity was assessed by flow cytometry. Immunofluorescence and immunohistochemistry were used to examine the level of NLRP3 in chondrocytes and rat cartilage, respectively. The progression of OA was monitored with hematoxylin-eosin (H&E) staining and safranin O/fast green staining.

resultsICA could suppress LPS-induced inflammation and reduction of collagen formation in chondrocytes. Furthermore, ICA could inhibit NLRP3 inflammasome-mediated caspase-1 signaling pathway to alleviate pyroptosis induced by LPS. Overexpression of NLRP3 reversed the above changes caused by ICA. It was further confirmed in the rat OA model that ICA alleviated OA by inhibiting NLRP3-mediated pyroptosis.

conclusionICA inhibited OA via repressing NLRP3/caspase-1 signaling-mediated pyroptosis in models of OA in vitro and in vivo, suggesting that ICA might be a promising compound in the treatment of OA.

Indexed as

AnimalsAnti-Inflammatory Agents, Non-SteroidalArthritis, ExperimentalCartilage, ArticularCells, CulturedChondrocytesCollagenDrug Evaluation, PreclinicalFlavonoidsInflammasomesLipopolysaccharidesMaleNLR Family, Pyrin Domain-Containing 3 ProteinOsteoarthritisPyroptosisSignal TransductionAnti-Inflammatory Agents, Non-SteroidalCollagenFlavonoidsicariinInflammasomesLipopolysaccharidesNLR Family, Pyrin Domain-Containing 3 ProteinNlrp3 protein, ratCaspase-1 signalingIcariinNLRP3 inflammasomeOsteoarthritisPyroptosis

Identifiers

PMID31511005
PMCPMC6737611
OpenAlexW2972897928

What OpenQuestion holds

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.